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Abstract

A novel actinomycete, designated strain TRM 66233, was isolated from L. collected from the Xinjiang Uygur Autonomous Region of China and characterized using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain TRM 66233 with the genus . Strain TRM 66233 showed a high similarity value to NRRL B-1049 (98.07 %) based on the 16S rRNA gene phylogenetic tree. The whole-cell sugar pattern of TRM 66233 consisted of glucose, galactose, mannose and ribose. The predominant menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and four unidentified lipids. The major fatty acids were -C, -C, -C, C and -C. The G+C content of the DNA was 70.35 mol%. The DNA–DNA relatedness and average nucleotide identity values as well as evolutionary distances based on multilocus (D, B, A, B and B) sequences between strain TRM 66233 and closely related type strains were significantly lower than the recommended threshold values. The whole-genome average nucleotide identity and digital DNA–DNA hybridization values between strain TRM 66233 and NRRL B-1049 were 78.86 and 23.2 %, respectively. On the basis of evidence from this polyphasic study, strain TRM 66233 should represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRM 66233 (=CCTCC AA 2019056=LMG 31559).

Funding
This study was supported by the:
  • Chuan-Xing Wan , Microbial Resources Utilization Innovation Team in the Key Field of Xinjiang Production and Construction Crops , (Award 2017CB014)
  • Lili Zhang , Key Projects of National Natural Science Foundation of China , (Award U1703236)
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2020-08-13
2020-11-25
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