1887

Abstract

A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, O9, was isolated from a forest soil sample collected at Dai, Xishuangbanna, Yunnan Province, PR China. Strain O9 grew optimally at pH 7.0, at 28‒30 °C and in the absence of NaCl. 16S rRNA gene sequence analysis placed strain O9 within the genus of the family with KP01 (97.8 %), JN53 (97.7 %), JS16-4 (97.4 %), JN246 (97.3 %) and DHOC24 (97.3 %) as its closest relatives. Strain O9 hydrolysed casein, gelatin and Tween 80. It could not assimilate -arabinose, -rhamnose, sucrose, melibiose, gentiobiose or -fructose as a carbon source. It was negative for esterase lipase (C8) and -glucosidase. Phosphatidylethanolamine was the predominant polar lipid. The major respiratory quinone of strain O9 was MK-7. Its major fatty acids were iso-C (34.2 %), C 5 (20.9 %) and iso-C 3-OH (12.6 %). The genomic DNA G+C content of strain O9 was 49.0 mol% based on total genome calculations. The average nucleotide identity score between the genomic sequence of strain O9 and that of KP01 was 72.9%. The Genome-to-Genome Distance Calculator showed that DNA‒DNA hybridization values for strain O9 and KP01 were 13.6, 21.1 and 14.4%, respectively. Based on the polyphasic taxonomic data, strain O9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is O9 (=CGMCC 1.12462=KCTC 32404).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award NSFC, grant number 31070002)
  • Jianli Zhang , National Key Research and Development Program of China , (Award 2016YFC0501302)
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2020-07-23
2020-09-20
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