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Abstract

A novel, obligately anaerobic bacterium (strain SURF-ANA1) was isolated from deep continental subsurface fluids at a depth of 1500 m below surface in the former Homestake Gold Mine (now Sanford Underground Research Facility, in Lead, South Dakota, USA). Cells of strain SURF-ANA1 were Gram-negative, helical, non-spore-forming and were 0.25–0.55×5.0–75.0 µm with a wavelength of 0.5–0.62 µm. Strain SURF-ANA1 grew at 15–50 °C (optimally at 40 °C), at pH 4.8–9.0 (pH 7.2) and in 1.0–40.0 g l NaCl (10 g l NaCl). The strain grew chemoheterotrophically with hydrogen or mono-, di- and polysaccharides as electron donors. The major cellular fatty acids in order of decreasing abundance (comprising >5% of total) were 10-methyl C, iso-C, C and C dimethyl acetal (DMA) and C methylene-nonadecanoic acid. Phylogenetic analysis based on the 16S rRNA gene sequence of strain SURF-ANA1 indicated a closest relationship with the recently characterized (99%). Despite high sequence identity, because of its distinct physiology, morphology and fatty acid profile, strain SURF-ANA1 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. To our knowledge, this is the first report of an isolate within the phylum from the deep (>100 m) terrestrial subsurface. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and genomic sequences of strain SURF-ANA1 are KU359248 and GCF 009768935.1, respectively. The type strain of is SURF-ANA1 (=ATCC TSD-67=JCM 32656).

Funding
This study was supported by the:
  • National Aeronautics and Space Administration (Award NNA13AA92A)
    • Principle Award Recipient: Jan Amend
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2020-07-30
2021-08-02
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