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Volume 70, Issue 8

sp. nov., isolated from a salt lake Free

Abstract

A novel Gram-stain-negative, strictly aerobic, gliding and rod-shaped bacterial strain, designated strain C33, was isolated from Yuncheng Salt Lake, Shanxi, PR China. Strain C33 grows optimally at 37 °C, pH 7.5 and 5.0 % (w/v) NaCl. Cells of strain C33 are 0.3–0.5 µm wide and 1.0–2.0 µm long, catalase-positive and oxidase-positive. The major cellular fatty acids are iso-C and iso-C. The sole respiratory quinone is Q-8. The major polar lipids include phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophospholipid, one unidentified glycolipid and four unidentified lipids. The results of phylogenetic analysis based on 16S rRNA gene sequences indicate that strain C33 has the highest similarities to KCTC 42284 (97.4 %), XDB06 (96.5 %) and ‘’ MCCC 1K03442 (95.2 %). The percentage of conserved proteins and average amino acid identity values between strain C33 and its close related species are higher than the threshold for dividing genera, the average nucleotide identity and digital DNA–DNA hybridization values are well below the threshold limits for species delineation. The genomic DNA G+C content is 63.7 mol%. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain C33 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C33 (=MCCC 1H00413=KCTC 72874).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , gene annotation , polyphasic taxonomy , proteobacteria , salt lake and Wenzhouxiangella
Funding
This study was supported by the:
  • Science and Technology Basic Resources Investigation Program (Award 2017FY100300)
    • Principle Award Recipient: Zong-Jun Du
  • National Natural Science Foundation of China (Award 31770002)
    • Principle Award Recipient: Zong-Jun Du
© 2020 The Authors
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2020-07-13
2024-04-27
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Wenzhouxiangella limi sp. nov., isolated from a salt lake
Int J Syst Evol Microbiol 70, 4610 (2020); https://doi.org/10.1099/ijsem.0.004320
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