1887

Abstract

Four isolates of two novel ascosporogenous species belonging to the clade were obtained from cactus tissues and rotting wood in Brazil. Results of analyses of the sequences of the ITS and D1/D2 domains of the large subunit rRNA gene indicated that the two isolates of the cactophilic species are related to and , yeasts that are associated with cacti and require an organic source of sulfur for growth. We propose the novel species sp. nov. (CBS 16113; MycoBank number: MB 834400) to accommodate these isolates. The other two isolates are phylogenetically related to and , also in the clade. The novel species name sp. nov. (CBS 16316; MycoBank number: MB 834402) is proposed for this species.

Funding
This study was supported by the:
  • Marc-André Lachance , the Natural Science and Engineering Council of Canada
  • Carlos A. Rosa , Fundação de Amparo à Pesquisa do Estado de Minas Gerais , (Award APQ-01525-14, APQ-01477-13 and APQ-02552-15)
  • Carlos A. Rosa , Conselho Nacional de Desenvolvimento Científico e Tecnológico , (Award 407415/2013-1 and 0457499/2014-1)
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2020-06-25
2020-08-06
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References

  1. Yamada Y, Higashi T, Ando S, Mikata K. The phylogeny of strains of species of the genus Pichia Hansen (Saccharomycetacea) based on the partial sequences of 18S ribosomal RNA: The proposals of Phaffomyces and Starmera, the new genera. Bull Fac Agric Shizuoka Univ 1997; 47:23–35
    [Google Scholar]
  2. Yamada Y, Kawasaki H, Nagatsuka Y, Mikata K, Seki T. The phylogeny of the cactophilic yeasts based on the 18S ribosomal RNA gene sequences: the proposals of Phaffomyces antillensis and Starmera caribaea, new combinations. Biosci Biotechnol Biochem 1999; 63:827–832 [CrossRef][PubMed]
    [Google Scholar]
  3. Kurtzman CP, Robnett CJ, Basehoar-Powers E. Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov. FEMS Yeast Res 2008; 8:939–954 [CrossRef][PubMed]
    [Google Scholar]
  4. Kurtzman CP, Yamada H. Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp 805–810
    [Google Scholar]
  5. Freitas LFD, Barbosa R, Sampaio JP, Lachance M-A, Rosa CA. Starmera pilosocereana sp. nov., a yeast isolated from necrotic tissue of cacti in a sandy coastal dune ecosystem. Int J Syst Evol Microbiol 2015; 65:4474–4478 [CrossRef][PubMed]
    [Google Scholar]
  6. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP. Candida Berkhout. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study 2011, 5th ed. Amsterdam: Elsevier; 1923 pp 987–1278
    [Google Scholar]
  7. Nakase T, Jindamorakot S, Am-in S, Potacharoen W, Tanticharoen M et al. Pichia nongkratonensis sp. nov., a new species of ascomycetous yeast isolated from insect frass collected in Thailand. Mycoscience 2005; 46:192–195 [CrossRef]
    [Google Scholar]
  8. Am-In S, Limtong S, Yongmanitchai W, Jindamorakot S. Candida andamanensis sp. nov., Candida laemsonensis sp. nov. and Candida ranongensis sp. nov., anamorphic yeast species isolated from estuarine waters in a Thai mangrove forest. Int J Syst Evol Microbiol 2011; 61:454–461 [CrossRef][PubMed]
    [Google Scholar]
  9. Shen X-X, Opulente DA, Kominek J, Zhou X, Steenwyk JL et al. Tempo and mode of genome evolution in the budding yeast subphylum. Cell 2018; 175:1533–1545 [CrossRef][PubMed]
    [Google Scholar]
  10. Morais CG, Batista TM, Kominek J, Borelli BM, Furtado C et al. Spathaspora boniae sp. nov., a d-xylose-fermenting species in the Candida albicans/Lodderomyces clade. Int J Syst Evol Microbiol 2017; 67:3798–3805 [CrossRef][PubMed]
    [Google Scholar]
  11. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp 87–110
    [Google Scholar]
  12. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications San Diego, CA: Academic Press; 1990 pp 315–322
    [Google Scholar]
  13. O'Donnell K. Fusarium and its near relatives. In Reynolds DR, Taylor JW. (editors) The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematic Oregon: CAB International; 1993 pp 225–233
    [Google Scholar]
  14. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [CrossRef][PubMed]
    [Google Scholar]
  15. Lachance MA, Bowles JM, Starmer WT, Barker JS. Kodamaea kakaduensis and Candida tolerans, two new ascomycetous yeast species from Australian Hibiscus flowers. Can J Microbiol 1999; 45:172–177 [CrossRef][PubMed]
    [Google Scholar]
  16. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  17. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [CrossRef][PubMed]
    [Google Scholar]
  18. Wickerham LJ, Burton KA. A simple technique for obtaining mating types in heterothallic diploid yeasts, with special reference to their uses in the genus Hansenula . J Bacteriol 1954; 67:303–308 [CrossRef][PubMed]
    [Google Scholar]
  19. Morais CG, Sena LMF, Lopes MR, Santos ARO, Barros KO et al. Production of ethanol and xylanolytic enzymes by yeasts inhabiting rotting wood isolated in sugarcane bagasse hydrolysate. Fungal Biol 2020 [CrossRef]
    [Google Scholar]
  20. Kurtzman CP. Description of new yeast species-is one strain enough?. Bull BISMiS 2010; 1:17–24
    [Google Scholar]
  21. Brysch-Herzberg M, Wohlmann E, Fischer R. Zygosaccharomyces seidelii sp. nov. a new yeast species from the Maldives, and a revisit of the single-strain species debate. Antonie van Leeuwenhoek 2020; 113:427–436 [CrossRef][PubMed]
    [Google Scholar]
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