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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 7

sp. nov., isolated from deep-sea sediment of the Pacific Ocean Free

Abstract

A taxonomic study was carried out on strain PA15-N-34, which was isolated from deep-sea sediment of Pacific Ocean. The bacterium was Gram-stain-positive, oxidase- and catalase-positive and rod-shaped. Growth was observed at salinity of 0–15.0% NaCl and at temperatures of 10–45 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PA15-N-34 belonged to the genus , with the highest sequence similarity to MTEO17 (97.7 %), followed by 19 m-6 (97.3 %) and 12 other species of the genus (93.4 %–97.0 %). The average nucleotide identity and DNA–DNA hybridization values between strain PA15-N-34 and type strains of the genus were 71.46–81.78% and 18.7–25.2 %, respectively. The principal fatty acids (>10 %) were summed feature 8 (C 7 and/or C 6; 31.2 %), C (25.0 %) and summed feature 3 (14.6 %). The DNA G+C content was 57.15 mol%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, four unidentified aminolipids and three unidentified lipids. The novel strain can be differentiated from its closest type strain by a negative test for urease and the presence of diphosphatidylglycerol and aminolipid. The combined genotypic and phenotypic data show that strain PA15-N-34 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain PA15-N-34 (=MCCC 1A14738=KCTC 72163).

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  • Accepted:
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Keyword(s): Alcanivorax sediminis sp. nov. , deep-sea sediment , Pacific Ocean and taxonomy
Funding
This study was supported by the:
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award (NIMR-2020-9))
    • Principle Award Recipient: Zongze Shao
  • Xiamen Ocean Economic Innovation and Development Demonstration Project (Award (16PZP001SF16))
    • Principle Award Recipient: Zongze Shao
  • National Natural Science Foundation of China (Award (No. 41976107, 91851203))
    • Principle Award Recipient: Zongze Shao
  • COMRA program (Award (No. DY135-B2-01, DY135-E2-5-02))
    • Principle Award Recipient: Zongze Shao
© 2020 The Authors
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2020-07-03
2024-04-26
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Alcanivorax sediminis sp. nov., isolated from deep-sea sediment of the Pacific Ocean
Int J Syst Evol Microbiol 70, 4280 (2020); https://doi.org/10.1099/ijsem.0.004285
/content/journal/ijsem/10.1099/ijsem.0.004285
/content/journal/ijsem/10.1099/ijsem.0.004285
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