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Volume 70, Issue 7

sp. nov., isolated from a saline–alkaline soil Free

Abstract

A novel moderately halophilic, filamentous actinobacterium, designated as XMNu-373, was isolated from a saline–alkaline soil sample collected from the Mongolia Plateau, Dongwu County, Inner Mongolia Autonomous Region, PR China. The isolate grew optimally at 28‒37 °C, pH 7.0‒8.0 and with 2–5 % (w/v) NaCl. The substrate mycelia fragmented into rod-like elements, and the white aerial mycelia formed spore chains at maturity. The predominant menaquinone was MK-9(H). The polar lipids were diphosphatidylglycerol, three unidentified phosphoglycolipids, an unidentified aminophospholipid, two phosphatidylinositol mannosides, four unidentified phospholipids, phosphatidylglycerol and two unidentified lipids. The major cellular fatty acids were iso-C, anteiso-C and anteiso-C. The genomic DNA G+C content was 66.2 mol%. It shared high 16S rRNA gene sequence similarities to YIM 96448 (96.1 %) and EGI 60009 (96.0 %). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain XMNu-373 resided in the clade of family , and it formed a monophyletic branch distinct from four other recognized type species in the subclade of the genus . On the basis of polyphasic taxonomic evidence, strain XMNu-373 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XMNu-373 (=JCM 33740=CGMCC 4.7654).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): actinobacteria , Phytoactinopolyspora mesophila , strain XMNu-373T and taxonomy
Funding
This study was supported by the:
  • National College Students Innovation and Entrepreneurship Training Program (Award 201910092005)
    • Principle Award Recipient: Xue-Han Zhai
  • Sino-Hungarian Bilateral S&T Cooperation Projects from 2019 to 2020 (Award Chinese Project: 8-11; Hungarian project: 2018-2.1.14-TÉT-CN-2018-00021)
    • Principle Award Recipient: Cheng-Hang Sun
  • Natural Science Foundation of Hebei Province (Award C2017405031)
    • Principle Award Recipient: Xiao-Jun Li
  • National Natural Science Foundation of China (Award 81703476)
    • Principle Award Recipient: Xiao-Jun Li
© 2020 The Authors
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2020-06-19
2024-04-26
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Phytoactinopolyspora mesophila sp. nov., isolated from a saline–alkaline soil
Int J Syst Evol Microbiol 70, 4179 (2020); https://doi.org/10.1099/ijsem.0.004274
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