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Volume 70, Issue 6

sp. nov., isolated from spinach farming field soil Free

Abstract

A Gram-stain-negative bacterium, designated strain YJ09, was isolated from spinach farming field soil at Shinan in the Republic of Korea. Cells of strain YJ09 were found to be strictly aerobic, non-motile, non-spore-forming creamy-yellow rods which can grow at 20–37 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0–8.0) and at salinities of 0–0.5 % (w/v) NaCl (optimum, 0 % NaCl). The 16S rRNA gene sequence analysis showed that strain YJ09 belongs to the genus with high sequence similarities to GP20-2 (98.0 %), HKS-06 (97.7 %) and JS5 (97.4 %). The results of phylogenetic analysis indicated that strain YJ09 formed a distinct phyletic line in the genus and the results of DNA–DNA relatedness studies demonstrated that strain YJ09 could be separated from its closest relatives in the genus . The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified glycolipids, an unidentified phospholipid and sphingoglycolipid. The predominant ubiquinone and polyamine components were Q-10 and spermidine, respectively. The major fatty acids were C ω7 C  and C ω7 and/or iso-C 2-OH. The DNA G+C content of this novel isolate was 65.9 mol%. On the basis of phenotypic, chemotaxonomic properties and phylogenetic analyses in this study, strain YJ09 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is YJ09 (=KACC 19551=NBRC 113247).

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  • Accepted:
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Keyword(s): rhizosphere , Sphingomonas segetis sp. nov. and taxonomy
Funding
This study was supported by the:
  • Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (as part of the Genome Technology to Business Translation Program) (Award 918016-4)
    • Principle Award Recipient: Kyung-Sook Whang
© 2020 The Authors
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2020-06-05
2024-05-13
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Sphingomonas segetis sp. nov., isolated from spinach farming field soil
Int J Syst Evol Microbiol 70, 3905 (2020); https://doi.org/10.1099/ijsem.0.004257
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