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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 6

sp. nov., isolated from a tidal flat Free

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, motile by single polar flagellum and ovoid or rod-shaped bacterial strain, designated JBTF-M18, was isolated from tidal-flat sediment collected from the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M18 fell within the clade comprising the type strains of species. Strain JBTF-M18 exhibited 16S rRNA gene sequence similarity values of 97.1–98.8 % to the type strains of , , and and of less than 96.9 % to the type strains of the other species. The average nucleotide identity and digital DNA–DNA hybridization values between strain JBTF-M18 and the type strains of and were 72.0 and 89.5% and 18.9 and 38.1 %, respectively. DNA–DNA relatedness values between strain JBTF-M18 and the type strains of and were 14 and 19 %, respectively. The DNA G+C content of strain JBTF-M18 from genomic sequence data was 52.9 %. Strain JBTF-M18contained MK-6 as the predominant menaquinone and Q-7 and Q-8 as the predominant ubiquinones. It had iso-C, summed feature 3 (C ω and/or C ω) and C as the major fatty acids. The major polar lipids of strain JBTF-M18 were phosphatidylethanolamine and phosphatidylglycerol. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain JBTF-M18 is separated from recognized species. On the basis of the data presented, strain JBTF-M18 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JBTF-M18 (=KACC 19869=NBRC 113583).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome , novel species , polyohasic taxonomy , Shewanella insulae and tidal flat
Funding
This study was supported by the:
  • Rural Development Administration (Award PJ013743)
    • Principle Award Recipient: Jung-Hoon Yoon
  • National Institute of Biological Resources (Award project on survey of indigenous species of Korea)
    • Principle Award Recipient: Jung-Hoon Yoon
© 2020 The Authors
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2020-06-08
2025-05-19
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Shewanella insulae sp. nov., isolated from a tidal flat
Int J Syst Evol Microbiol 70, 3872 (2020); https://doi.org/10.1099/ijsem.0.004252
/content/journal/ijsem/10.1099/ijsem.0.004252
/content/journal/ijsem/10.1099/ijsem.0.004252
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