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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 6

sp. nov., a marine bacterium isolated from hadal seawater of the Mariana Trench Free

Abstract

A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterium, designated ZYF650, was isolated from the hadal seawater (9600 m) of the Mariana Trench. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that ZYF650 formed a lineage within the family that was distinct from the most closely related species and with 16S rRNA gene sequences similarities of 98.0 and 97.7 %, respectively. Strain ZYF650 showed average nucleotide identity values of 75.7 % with , 73.3 % with and 79.3 % with , and DNA–DNAhybridization values of 21.5, 21.3 and 22.0 % with , and , respectively, which were lower than the threshold for species delineation. Strain ZYF650 grew with 0–14 % (w/v) NaCl (optimum, 7–8 %) at a temperature range of 10–45 °C (optimum, 28 °C) and pH 6.0–9.5 (optimum, pH 7.0–8.0). The sole respiratory quinone was ubiquinone-9 (Q-9). The polar lipids in ZYF650 comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unidentified polar lipids, two unidentified aminolipids and two phospholipids. The predominant fatty acids (more than 10 % of total fatty acids) were C ω9 (21.9 %), C (21.7 %), C 3-OH (14.0 %), C ω9 (13.2 %) and C (12.2 %). The DNA G+C content of strain ZYF650 was 55.6 %. On the basis of polyphasic taxonomic analysis, strain ZY650 is considered to represent a novel specie of the genus in the family , for which the name sp. nov. is proposed. The type strain is ZYF650 (=JCM 33013=MCCC 1K03552).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): hadal seawater , Mariana Trench and Marinobacter salinexigens
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41730530)
    • Principle Award Recipient: Xiao-Hua Zhang
  • National Natural Science Foundation of China (Award 91751202)
    • Principle Award Recipient: Xiao-Hua Zhang
© 2020 The Authors
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2020-05-22
2024-05-12
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Marinobacter salinexigens sp. nov., a marine bacterium isolated from hadal seawater of the Mariana Trench
Int J Syst Evol Microbiol 70, 3794 (2020); https://doi.org/10.1099/ijsem.0.004236
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