1887

Abstract

A polyphasic taxonomic study was performed on an unidentified -like Gram-stain-positive bacterium designated strain C605018/01/1 isolated from a milk sample collected from the udder of a cow at . Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus and was most closely related to the type strain of (99.76 %); sequence similarities to all other species were below 97 %. The wet-lab DNA–DNA hybridization values among strain C605018/01/1 and DSM 13483ᵀ were low, 16.9 % (reciprocal, 49.8 %). Pertaining to the whole genome sequence with a total length of 2.02 Mb and 1654 protein counts, the novel strain C605018/01/01 displayed a G+C content of 51.6 % mol%. The presence of the major menaquinone MK-9(H) supported the affiliation of this strain to the genus . The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol-mannoside and unidentified glycolipid and aminophospholipids. Based on these results it is proposed that strain C605018/01/1 should be classified as representing a novel species, sp. nov. The type strain C605018/01/1 (CCUG 45425=DSM 107286=BCCM/LMG 30783)

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2020-06-26
2020-07-02
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