1887

Abstract

A Gram-stain-negative, aerobic, gliding, rod-shaped (0.2–0.5×1.0-13.0 µm) and yellow-pigmented bacterium, designated PLHSN227, was isolated from seawater collected near the coast of Yantai, PR China. PLHSN227 was found to grow at 15–37 °C (optimum, 28–30 °C) and pH 6.0–8.5 (optimum, 6.5–7.5) in the presence of 2–14 % (w/v) NaCl (optimum, 5.0 %). Phylogenetic analysis of the 16S rRNA gene sequences revealed that PLHSN227 represented a member of the family and exhibited the highest sequence similarity (94.6 %) to the type strain NBRC 100249. The chemotaxonomic analysis revealed that the sole respiratory quinone was menaquinone 6 (MK-6) and the major fatty acids included Cω8, iso-C, anteiso-C, C and summed feature 8 (Cω7 and/or Cω6). The major polar lipids included phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content of PLHSN227 was 35.6 mol%. PLHSN227 showed the highest average amino acid identity value of 67.2 %, the average nucleotide identity value of 75.6 and 14.5 % digital DNA–DNA hybridization identity with DSM 15361. According to the phylogenetic data, PLHSN227 formed a distinct clade in the phylogenetic tree. On the basis of phenotypic, chemotaxonomic and phylogenetic data, it is considered that PLHSN227 represents a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is PLHSN227 (=KCTC 72159=MCCC 1H00371).

Funding
This study was supported by the:
  • Zong-Jun Du , the National Natural Science Foundation of China , (Award 31770002)
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2020-05-22
2020-06-03
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