1887

Abstract

Two Gram-stain-negative, short rod-shaped and non-flagellated strains, designated 17-4A and L52-1-41, were isolated from the surface seawater of the Indian Ocean and South China Sea, respectively. The 16S rRNA genes of the two strains shared sequence similarity of 99.45 %. Strain 17-4A shared the highest 16S rRNA gene similarity of 98.02 % with EBR-8-1, followed by BN9 (97.47 %), MJ07 (96.93 %), Ch07 (96.68 %), DCY25 (96.65 %), PB3-7B (96.63 %), 24 (96.34 %), 54Pin (96.28 %) and B201 (96.05 %). L52-1-41 shared the highest 16S rRNA gene similarity of 97.74 % with EBR-8-1, followed by BN9 (97.47 %), MJ07 (96.65 %), Ch07 (96.41 %), DCY25 (96.37 %), PB3-7B (96.35 %), B201 (96.28 %), and 24 (96.06 %). The results of phylogenetic analyses indicated that 17-4A and L52-1-41 formed a stable, distinct and highly supported lineage affiliated to the genus . The results of the digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses indicated that they represented a single species. They featured similar genomic DNA G+C contents of 53.2–53.4 mol%. Activities of catalase and oxidase were negative for both strains. The fatty acids patterns of 17-4A and L52-1-41 were most similar, mostly comprised of C, Ccyclo, C, Cω9 and summed feature 8 (Cω7 and/or C ω6). The major polar lipids of the two strains were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified aminolipids. The respiratory quinone of the two strains was Q-8. Hence, on the basis of the phenotypic, chemotaxonomic and genotypic data presented in this study, we proposed the classification of both strains as representatives of a novel species named sp. nov., with the type strain 17-4A (=MCCC 1A12670=KCTC 62121=NBRC 113794), and another strain L52-1-41 (=MCCC 1A05046=KCTC 52313).

Funding
This study was supported by the:
  • Zongze Shao , National Infrastructure of Microbial Resources of China , (Award NIMR-2019-9)
  • Zongze Shao , NSFC Hydrosphere Project , (Award 91851203)
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2020-05-05
2020-06-04
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