1887

Abstract

A novel Gram-staining-negative, spiral-shaped bacterium, designated strain 64-1, was isolated from oil reservoir water collected from Liaohe oilfield, north-eastern China. Growth occurred at 15–55 °C and pH 6.0–10.0. The sole respiratory quinone was Q-10. The predominant cellular fatty acids were summed feature 8 (C 7 /C 6), C and C cyclo 8. The polar lipids consisted of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), an unidentified aminophospholipid (UAPL), an unidentified aminolipid (UAL) and two unidentified polar lipids (UPL). The genomic DNA G+C content of strain 64-1 was 64.5 mol%. Strain 64-1 shared the highest 16S rRNA gene sequence similarities with JA145 (92.0 %) and 26-4b1 (91.8 %). In the phylogenetic trees, the strain constituted a sub-cluster within the family . Based on the results of morphological, physiological, biochemical and phylogenetic analysis, strain 64-1 represents a new species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is 64-1 (=CGMCC 1.16798=LMG 31399).

Funding
This study was supported by the:
  • Lei Wang , National Natural Science Foundation of China , (Award Grant No. 41977198)
  • , National Science and Technology Major Project , (Award 2016ZX05040002-005-001)
  • Lei Wang , Methodological research and application of oil field ecological profit and loss assessment , (Award 2016E-1205)
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2020-05-05
2020-06-02
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