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Volume 70, Issue 5

sp. nov., isolated from yellow water of strongly flavored Chinese baijiu Free

Abstract

A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China). Oval endospores were formed at the subtermini of cells with swollen sporangia. The isolate was able to grow at temperatures of 20–45 °C (optimum growth at 37 °C), at pH 6.0–10.0 (optimum growth at pH 8.0) and in the presence of 0–2 % (w/v) NaCl (optimum growth with 0 % NaCl). Ribose was the major cell-wall sugar, and -diaminopimelic acid (-DAP) was the diagnostic amino acid. The main polar lipids of 3 H-10 included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). MK-7 was predominant menaquinone and iso-C (60.7 %) was the major fatty acid. Comparisons of 16S rRNA gene sequence indicated that 3 H-10 was most closely related to SA4 (96.30 %), Gsoil 114 (96.27 %) and LMG 18435 (96.27 %). The average nucleotide identity (ANI) values between strain 3 H-10 and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively. The genomic DNA G+C content was 35.4 mol%. On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 3 H-10 (=CICC 24755=JCM 33703).

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  • Accepted:
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  • Published Online:
Keyword(s): Bacillus aquiflavi , Chinese baijiu and Yibin region
Funding
This study was supported by the:
  • Open Foundation of Key Laboratory of Wuliangye -flavor Liquor Solid-state Fermentation, China National Light Industry (Award 2018JJ022)
    • Principle Award Recipient: Lei Zhai
© 2020 The Authors
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2020-05-04
2024-04-26
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Bacillus aquiflavi sp. nov., isolated from yellow water of strongly flavored Chinese baijiu
Int J Syst Evol Microbiol 70, 3406 (2020); https://doi.org/10.1099/ijsem.0.004185
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