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Volume 70, Issue 5

sp. nov., a novel actinobacterium isolated from soil Free

Abstract

A novel actinobacterium, designated strain H14505, was isolated from a soil sample collected in Hong Yuan, Sichuan, southwest PR China. The temperature, pH and NaCl ranges for growth were determined to be 15–35 °C (optimum, 28 °C), 6.0–8.0 (optimum, pH 7.0) and 0–2 % (w/v; optimum without NaCl), respectively. The polar lipdis detected for strain H14505 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, glycolipid and four unidentified lipids. The predominant menaquinones of strain H14505 were MK-9(H) and MK-9(H), and the prevalent fatty acids (>10 %) were C ω9, C ω8, summed feature 5 (anteiso-C/ C ω6,9) and C. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences indicated that strain H14505 showed high similarity to NEAU-JM1 (99.0 %) and NEAU-CL2 (99.0 %), and formed a monophyletic clade within the the genus in the phylogenetic trees. However, the average nucleotide indentity and DNA–DNA hybridization values between strain H14505 and closely related species showed that it belonged to a distinct species. Furthermore, the results of morphological, physiological and biochemical tests allowed further phenotypic differentiation of strain H14505 from its closest relatives. Thus, it is proposed that strain H14505 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is H14505 (=JCM 32394=CICC 11042).

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  • Accepted:
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  • Published Online:
Keyword(s): 16S rRNA gene , Catellatospora sichuanensis sp. nov and polyphasic taxonomy
Funding
This study was supported by the:
  • National R&D Infrastructure and Facility Development Program of China (Award 2019JDPT0012)
    • Principle Award Recipient: Yiwen Chu
  • Fund of shared Platform of Sichuan Medical Microorganism (Award 2018TJPT0004)
    • Principle Award Recipient: Yidong Guo
  • Start-up fund of introducing talent of Chengdu University (Award 2081915049)
  • Sicence and Technology Bureau Program of Chengdu Municipal Government (Award 2016-XT00-00023-GX)
    • Principle Award Recipient: Yiwen Chu
© 2020 The Authors
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2020-04-29
2024-04-26
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Catellatospora sichuanensis sp. nov., a novel actinobacterium isolated from soil
Int J Syst Evol Microbiol 70, 3309 (2020); https://doi.org/10.1099/ijsem.0.004175
/content/journal/ijsem/10.1099/ijsem.0.004175
/content/journal/ijsem/10.1099/ijsem.0.004175
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