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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 5

sp. nov., isolated from the surface of a tomato Free

Abstract

A novel bacterial strain, designated TOUT106, was isolated from the surface of a tomato. The cells were rod-shaped, Gram-negative, encapsulated and non-motile. Strain TOUT106 grows best at 28 °C and pH 7.0 and can tolerate up to 2 % (w/v) NaCl. Based on 16S rRNA gene phylogeny, strain TOUT106 was placed close to the clade, with close similarity to subsp. strain NCTC 8297 (98.42 %). Results of genome-based phylogenetic analysis revealed that strain TOUT106 is placed well in the clade, by forming a distinct branch with DSM25444, NCTC132727, 06D021 and SB6412. The genomic DNA G+C content of strain TOUT106 is 53.53 mol%. The average nucleotide identity values of TOUT106 were less than 86.5 % with closely related members of the family . The major fatty acids of strain TOUT106 were C, Ccyclo, C3OH/Ciso, C, Ccyclo ω8, Cω6/C ω7, C and Cω7/C ω6. Strain TOUT106 showed differences in physiological, phenotypic and protein profiles by MALDI-TOF MS compared to its closest relatives. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain TOUT106 could be distinguished from the recognized species of the genus . It is suggested to represent a novel species of this genus, for which the name sp. nov. is proposed. The type strain is TOUT106 (=MCC 2901=KACC 21384=JCM 33718).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): genome phylogeny , Klebsiella , MALDI-TOF MS-based identification and nitrogen fixation
Funding
This study was supported by the:
  • Department of Biotechnology, Government of India (IN) (Award BT/Coord. II/01/03/2016)
    • Principle Award Recipient: Not Applicable
© 2020 The Authors
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2020-04-24
2025-03-25
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Klebsiella indica sp. nov., isolated from the surface of a tomato
Int J Syst Evol Microbiol 70, 3278 (2020); https://doi.org/10.1099/ijsem.0.004168
/content/journal/ijsem/10.1099/ijsem.0.004168
/content/journal/ijsem/10.1099/ijsem.0.004168
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