1887

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Volume 70, Issue 5

sp. nov. a novel plant growth-promoting bacterium with potential use in phytoremediation Open Access

Abstract

Strain RA15 was isolated from the rhizosphere of the halophyte plant growing in the Odiel marshes (Huelva, Spain). RA15 cells were Gram stain-negative, non-spore-forming, aerobic rods and formed cream-coloured, opaque, mucoid, viscous, convex, irregular colonies with an undulate margin. Optimal growth conditions were observed on tryptic soy agar (TSA) plates supplemented with 2.5 % NaCl (w/v) at pH 7.0 and 28 °C, although it was able to grow at 4–32 °C and at pH values of 5.0–9.0. The NaCl tolerance range was from 0 to 15 %. The major respiratory quinone was Q8 but Q9 was also present. The most abundant fatty acids were summed feature 3 (C ω7 and/or C ω6), C 8 and C. The polar lipids profile comprised phosphatidylglycerol and phosphatidylethanolamine as the most abundant representatives. Phylogenetic analyses confirmed the well-supported affiliation of strain RA15 within the genus , close to the type strains of , and . Results of comparative phylogenetic and phenotypic studies between strain RA15 and its closest related species suggest that RA15 could be a new representative of the genus , for which the name sp. nov. is proposed. The type strain is RA15 (=CECT 9079=LMG 29860). The whole genome has 5.3 Mb and the G+C content is 40.4 mol%.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Arthrocnemum macrostachyum , heavy metals , nitrogen fixation , odiel marshes and rhizosphere
Funding
This study was supported by the:
  • Newcastle University (Award Postdoctoral scholarship)
    • Principle Award Recipient: Lorena Carro
  • Instituto Nacional de Investigaciones Agropecuarias (Award RTA 2012-0006-C03-03 project)
    • Principle Award Recipient: Salvadora Navarro-Torre
  • Junta de Andalucía (Award P11-RNM-7274MO project)
    • Principle Award Recipient: Salvadora Navarro-Torre
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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Pseudoalteromonas rhizosphaerae sp. nov., a novel plant growth-promoting bacterium with potential use in phytoremediation
Int J Syst Evol Microbiol 70, 3287 (2020); https://doi.org/10.1099/ijsem.0.004167
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