1887

Abstract

A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435, was isolated from soil sample collected from the Cholistan Desert, Pakistan. The taxonomic position of the strain was established by using a polyphasic taxonomic approach. The cells were coccoid-shaped and found in single or arrangement of pairs. The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7–8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %). Results of analysis based on 16S rRNA gene sequences showed that MON 2.2 was its closest relative with 97.4 % similarity followed by CPCC 204711 (95.2 %). In phylogenetic trees, strain F435 formed a robust cluster with the only member of the genus . The peptidoglycan isomer present in the cell wall was -diaminopimelic acid. The major fatty acid was determined to be anteiso-C. Characteristic polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipids and glycolipids. The predominant menaquinone was MK-9(H). The genomic G+C content was calculated as 73.5 mol%. The digital DNA–DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435 and MON 2.2 were 24.6 and 81.8 %, respectively. Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435 represents a novel specie of the genus , for which the name sp. nov. is proposed. The type strain is F435 (=JCM 33648=CGMCC 1.17443). The description of the genus has also been emended.

Funding
This study was supported by the:
  • Shao-Wei Liu , National Natural Sciences Foundation of China , (Award 81621064)
  • Shao-Wei Liu , Drug Innovation Major Project of China , (Award 2018ZX09711001-007-001)
  • Cheng-Hang Sun , CAMS innovation Fund for Medical Sciences , (Award CAMS 2017-I2M-B&R-08)
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2020-04-17
2020-06-04
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