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Volume 70, Issue 5

, a novel species isolated from rectal contents of Tibetan Plateau pika () Free

Abstract

Two Gram-stain-negative, strictly aerobic, bright-yellow-pigmented and rod-shaped bacteria (strains 100069 and 100111) with a single polar flagellum were isolated from the rectal contents of plateau pika (). Based on the results of nearly full-length 16S rRNA gene sequence and phylogenetic analyses, strains 100069 and 100111 belong to the genus , and are closest to 4-12 (98.02 % similarity), B9 (97.8 %) and THG-MD21 (97.74 %). The DNA G+C contents of these two isolates were 68.30 mol% and 68.29 mol%, respectively. The highest average nucleotide identity (ANI) value between strain 100111 and its closely related species was 83.34 %, well below the threshold of 95–96 %. The major cellular fatty acids were iso-C, iso-C and iso-C ω9. Polar lipid content was dominated by diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. Ubiquinone-8 (Q-8) was the predominant respiratory quinone. These two isolates grew optimally at 35–37 °C, pH 7.0–8.0 and with 1.0 % (w/v) NaCl. The results of ANI analysis and other characteristics obtained from our polyphasic study showed that strains 100069 and 100111 represent a novel species in genus , for which the name sp. nov. (type strain 100111=DSM 104077=CGMCC 1.16429) is proposed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ANI , Luteimonas , novel species and phylogenetic analysis
© 2020 The Authors
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2020-04-20
2024-05-13
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Luteimonas chenhongjianii, a novel species isolated from rectal contents of Tibetan Plateau pika (Ochotona curzoniae)
Int J Syst Evol Microbiol 70, 3186 (2020); https://doi.org/10.1099/ijsem.0.004151
/content/journal/ijsem/10.1099/ijsem.0.004151
/content/journal/ijsem/10.1099/ijsem.0.004151
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