1887

Abstract

A bacterial strain designated CAR-16 was isolated from a freshwater lake in Taiwan and characterized using the polyphasic taxonomic approach. Cells were Gram-stain-negative, aerobic, motile by gliding, rod-shaped and formed rose-colored colonies. Optimal growth occurred at 30 °C, pH 7 and with 0 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that CAR-16 represented a member of the family and formed a phylogenetic lineage in the genus . CAR-16 was most closely related to 59G-WUEMPEL with a 99.7 % 16S rRNA gene sequence similarity. CAR-16 showed 71.2–79.5 % average nucleotide identity and 17.8–21.7 % digital DNA–DNA hybridization identity with the strains of other species of the genus . The major fatty acids of strain CAR-16 were iso-C, iso-C 3-OH, Cω5 and summed feature 3 (Cω/Cω6 and/or iso-C 2-OH). The polar lipid profile consisted of a mixture of phosphatidylethanolamine and several uncharacterized aminophospholipids, phospholipids and lipids. The major isoprenoid quinone was MK-7. The genomic DNA G+C content of CAR-16 was 38.8 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, CAR-16 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CAR-16 (=BCRC 81153=LMG 30923=KCTC 62869).

Keyword(s): Aquirufa , freshwater and novel species
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2020-04-08
2020-06-04
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