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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 5

sp. nov., isolated from Arctic surface sediment Free

Abstract

A Gram-stain-negative, aerobic, non-motile, non-gliding, yellow-pigmented and rod-shaped bacterial strain, designated 1KV19, was isolated from a surface sediment sample collected near a bay in the Arctic. Growth of strain 1KV19 occurred in 1–4 % (w/v) NaCl (optimum, 2 %), at 4–35 °C (optimum, 25–30 °C) and at pH 6.5–8.0 (optimum, pH 7.0–7.5). The phylogenetic trees based on the 16S rRNA gene sequences showed that strain 1KV19 was associated with the genus and had the highest 16S rRNA gene sequence similarity to 325-5 with 98.1 % similarity. Similarity values between strain 1KV19 and the type strains of other species were in the range 95.9–97.6 %. The average nucleotide identity and digital DNA–DNA hybridization values between strain 1KV19 and related species of the genus were 76.4–79.1 and 19.9–22.3 %, respectively. The major cellular fatty acids of strain 1KV19 were iso-C 3-OH, iso-C and iso-C H. The respiratory quinone was MK-6. The major polar lipids of strain 1KV19 were phosphatidylethanolamine, one unidentified aminolipid and two unidentified polar lipids. The phenotypic, genotypic and chemotaxonomic differences between strain 1KV19 and its phylogenetic relatives indicate that strain 1KV19 should be regarded as representing a novel species in the genus , for which the name sp. nov. is proposed. The type strain is 1KV19 (=KCTC 62595=MCCC 1H00307).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 16S rRNA gene , dDDH , draft genome sequencing and Lutibacter
Funding
This study was supported by the:
  • Science & Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: Zong-Jun Du
  • SOA Key Laboratory for Polar Science, Polar Research Institute of China (Award KP201705)
    • Principle Award Recipient: Zong-Jun Du
© 2020 The Authors
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2020-04-17
2024-05-07
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Lutibacter citreus sp. nov., isolated from Arctic surface sediment
Int J Syst Evol Microbiol 70, 3154 (2020); https://doi.org/10.1099/ijsem.0.004146
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