1887

Abstract

Chiou . 2018 and McFrederick . 2018 are closely related, and they share 100 % 16S rRNA gene sequence similarity, 99.6 % gene sequence similarity, 100 % gene sequence similarity, 97.3 % average nucleotide identity (ANI) value and 76.6 % DNA–DNA hybridization (DDH) value, indicating that they represent the same species. Fatty acid methyl esters (FAME) analysis and phenotypic characterization also indicated that and are very similar. We propose Chiou . 2018 as a later heterotypic synonym of McFrederick . 2018. The taxonomic position of subsp. in the group was re-examined using a polyphasic approach, including sequence analyses of 16S rRNA, , and genes, average nucleotide identity analysis, DNA–DNA hybridization, fatty acid methyl ester analysis and phenotypic characterization. Results of 16S rRNA gene sequence analysis indicated that subsp. was closely related to subsp. , and in the group, sharing 99.6–99.7 % 16S rRNA gene sequence similarities. Results of , and gene sequence analyses indicated that subsp. was most closely related to subsp. , having 91.8 % gene sequence similarity, 98.9 % gene sequence similarity and 93.1 % gene sequence similarity. subsp. DSM 16365 shared 95.6 % ANI value and 62.9 % DDH value with subsp. ATCC 14917. The low DDH value confirmed that subsp. and subsp. represent two different species, rather than two different subspecies in the group. On the basis of the data from polyphasic characterization obtained in the present study and in previous studies, subsp. is elevated to the species level and represents a novel species of the genus , for which the name sp. nov. is proposed and the type strain is DKO 22 (=CIP 108320=DSM 16365=JCM 16169). Two novel Gram-stain-positive bacterial strains, designated 1206-1 and F027-1-2, were isolated from traditional pickle in Heilongjiang Province, PR China, and from the intestinal tract of a honey bee () in Hubei Province, PR China, respectively. The two bacteria were characterized by a polyphasic approach, including 16S rRNA gene sequence analysis, gene sequence analysis, gene sequence analysis, fatty acid methyl ester analysis, average nucleotide identity analysis, DNA–DNA hybridization analysis and an analysis of phenotypic features. The results of 16S rRNA gene sequence analysis indicated that strains 1206-1 and F027-1-2 were distantly related to , , , , , , , , , and . Strain 1206-1 exhibited 94.2–96.4 % 16S rRNA gene sequence similarities, 69.5–83.3 %  gene sequence similarities and 73.1–90.3 %  gene sequence similarities to type strains of phylogenetically related species. ANI and DDH values between strain 1206-1 and the type strains of phylogenetically related species were 52.7–73.7 % and 21.1–30.1 %, respectively. On the basis of the data obtained in the present study, a novel species, sp. nov. is proposed and the type strain is 1206-1 (=CCM 8981=CCTCC AB 2019200=LMG 31620).

Funding
This study was supported by the:
  • Chun Tao Gu , National Natural Science Foundation of China , (Award no. 31471594)
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2020-04-06
2020-06-04
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