1887

Abstract

A novel marine actinomycete, designated strain KJ-029, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan. 16S rRNA gene sequence analysis revealed that the new isolate was most closely related to LHW 63014 (99.3 % similarity). Phylogenetic analyses of the genus based on 16S rRNA gene sequences showed that strain KJ-029 was clustered with LHW 63014 and 202201. However, digital DNA–DNA hybridization analyses presented low levels of relatedness in the range of 24.8–32.9 % between strain KJ-029 and the above closely related strains. The novel strain contained -diaminopimelic acid and 3-OH-diaminopimelic acid, -glutamic acid, glycine and -alanine in the cell-wall peptidoglycan. The acyl type of the peptidoglycan was glycolyl and mycolic acids were absent. The major menaquinone was MK-9(H). The whole-cell sugars consisted of glucose, mannose, xylose and ribose. Phosphatidylethanolamine was detected as the major phospholipid and corresponded to phospholipid type II. The predominant cellular fatty acid was iso-C. The DNA G+C content of the genomic DNA was 71.5 mol%. Based on the present polyphasic study, strain KJ-029 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KJ-029 (=NBRC 113519=TBRC 9233).

Funding
This study was supported by the:
  • Bungonsiri Intra , Institute for Fermentation, Osaka
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2020-03-30
2020-06-04
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