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Volume 70, Issue 5

sp. nov., isolated from Blume Free

Abstract

A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004, was isolated from the tubers of Blume collected from Yunnan Province, PR China. The 16S rRNA gene sequence result showed that the strain SYSUP0004 shared low similarity (97.7 %) with the type strain of . SYSUP0004 grew at pH 6.0–9.0 (optimum, pH 8.0), temperature 4–30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl). The cell-wall peptidoglycan type was A4 with an interpeptide bridge -ornithine-glutamic acid. Cell-wall sugars were mannose, ribose, glucose, galactose and fucose. The menaquinone was MK-9(H). The major fatty acids were anteiso-C anteiso-C A, C and anteiso-C. The polar lipids of SYSUP0004 were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid. The genomic DNA G+C content was 76.5 %. The average nucleotide identity values between SYSUP0004 and members of the genus were below the cut-off level (95–96 %) recommended as the ANI criterion for interspecies identity. Thus, based on the above results strain SYSUP0004 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain, SYSUP0004 (=KCTC 49025=CGMCC 1.16405).

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Cellulomonas endophytica , endophyte , Gastrodia elataBlume and genome analysis
Funding
This study was supported by the:
  • The Science Foundation of Kunming Medical University Haiyuan college (Award 2018HY008 and 2019HY003)
    • Principle Award Recipient: Yan-Qiong Li
  • Guangdong Province Science and Technology Innovation Strategy Special Fund (Award 2018B020206001)
    • Principle Award Recipient: Yan-Qiong Li
© 2020 Sun Yat-Sen University, P.R. China
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2020-04-02
2024-04-26
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Cellulomonas endophytica sp. nov., isolated from Gastrodia elata Blume
Int J Syst Evol Microbiol 70, 3091 (2020); https://doi.org/10.1099/ijsem.0.004133
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