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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 5

sp. nov., a pink-coloured organism isolated from cheese made of cow’s milk Free

Abstract

A pink-coloured bacterium (strain KR32) was isolated from cheese and assigned to the ‘ group’. Members of the ‘pink group’ form a stable clade (100 % bootstrap value) and contain the species , and , which share ≥99.0 % 16S rRNA gene sequence similarity. Isolate KR32 showed highest 16S rRNA gene sequence similarity (99.9 %) to DSM 20550. Additional multilocus sequence comparison confirmed the assignment of strain KR32 to the clade ‘pink group’. Average nucleotide identity and digital DNA–DNA hybridization values between isolate KR32 and DSM 20550 were 82.85 and 26.30 %, respectively. The G+C content of the genomic DNA of isolate KR32 was 69.14 mol%. Chemotaxonomic analysis determined anteiso-C as the predominant fatty acid and MK-9(H) as the predominant menaquinone. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and monoacyldimannosyl-monoacylglycerol. The peptidoglycan type of the isolate was A3α. The carotenoid bacterioruberin was detected as the major pigment. At 10 °C, strain KR32 grew with increased concentrations of bacterioruberin and production of unsaturated fatty acids. Strain KR32 was a Gram-stain-positive, catalase-positive, oxidase-positive and coccus-shaped bacterium with optimal growth at 27–30 °C and pH 8. The results of phylogenetic and phenotypic analyses enabled the differentiation of the isolate from other closely related species of the ‘pink group’. Therefore, strain KR32 represents a novel species for which the name sp. nov. is proposed. The type strain is KR32 (=DSM 109896=LMG 31480=NCCB 100733).

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Keyword(s): Arthrobacter , bacterioruberin , cheese , cold-adapted , Micrococcaceae and novel species
© 2020 The Authors
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2020-03-30
2024-05-06
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Arthrobacter bussei sp. nov., a pink-coloured organism isolated from cheese made of cow’s milk
Int J Syst Evol Microbiol 70, 3027 (2020); https://doi.org/10.1099/ijsem.0.004125
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