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Volume 70, Issue 4

sp. nov., isolated from marine sediment of southern Scott Coast, Antarctica Free

Abstract

A novel bacterium, designated JB02H27, was isolated from marine sediment collected from the southern Scott Coast, Antarctica. Cells were Gram-stain-negative, facultatively anaerobic, polar-flagellated and motile rods. Growth occurred at 4–45 °C, at pH 7.0–9.0 and with 3–25 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain JB02H27 consistently fell within the genus and formed a clade together with DG893 (98.8 % similarity), KCTC 42705 (98.4 %), R9SW1 (98.4%) and CP12 (97.9 %), which were subsequently used as reference strains for comparisons of phenotypic and chemotaxonomic characteristics. Average nucleotide identity values between strain JB02H27 and the four related type strains were 80.9, 76.6, 81.9 and 76.3 %, respectively. The major fatty acids were summed feature 3, C, C 9 and C N alcohol. The polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and an unidentified phospholipid, aminolipid, aminophospholipid and glycolipids. The sole respiratory quinone was ubiquinone-9. The DNA G+C content was 56.9 mol%. Based on the genomic, phylogenetic, phenotypic and chemotaxonomic analysis, we propose that strain JB02H27 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JB02H27 (=GDMCC 1.1528=KCTC 62941).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): JB02H27 , marine sediment , Marinobacter and southern Scott Coast
Funding
This study was supported by the:
  • GDAS' Special Project of Science and Technology Development (Award 2020GDASYL-20200302002)
    • Principle Award Recipient: Ming-Xia Zhang
  • Science and Technology Plan Project of Guangzhou (Award 201704020088)
    • Principle Award Recipient: Hong-Hui Zhu
  • Science and Technology Plan Project of Guangdong (Award 2019B030316009, 2018A030313476)
    • Principle Award Recipient: Hong-Hui Zhu
  • Pearl River S&T Nova Program of Guangzhou (Award 201806010065)
    • Principle Award Recipient: An-Zhang Li
  • Guangdong Province Science and Technology Innovation Strategy Special Fund (Award 2019B020215001)
    • Principle Award Recipient: Ming-Xia Zhang
© 2020 The Authors
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2020-03-26
2024-04-26
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Marinobacter denitrificans sp. nov., isolated from marine sediment of southern Scott Coast, Antarctica
Int J Syst Evol Microbiol 70, 2918 (2020); https://doi.org/10.1099/ijsem.0.004120
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