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Abstract

A novel bacterial strain, designated KMB7, isolated from a freshwater pond in Taiwan, was characterized using a polyphasic taxonomic approach. Cells were Gram-stain-negative, motile by means of a single polar flagellum, rod-shaped and formed cream colonies. Optimal growth occurred at 25 °C, pH 7, and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain KMB7 is affiliated with species in the genus . The 16S rRNA gene sequence similarity indicated that strain KMB7 is closely related to species within the genus (95.2–97.6 % sequence similarity) and is most similar to CS-6 (97.6 %), followed by B6 (97.5 %). The average nucleotide identity and digital DNA–DNA hybridization identity between strain KMB7 and the closely related strains were 74.6–78.0 % and 19.0–21.2 %, respectively. The major fatty acids of strain KMB7 were summed feature 3 (C ω and/or C ω), C ω and C. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and four unidentified phospholipids. The sole isoprenoid quinone was ubiquinone-8 (Q-8). Genomic DNA G+C content of strain KMB7 was 65.4 %. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain KMB7 should be classified in a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KMB7 (=BCRC 81156=LMG 30924=KCTC 62867).

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2020-03-26
2020-03-31
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