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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 4

sp. nov., isolated from subtropical forest soil Free

Abstract

A novel bacterial strain, designated FGD1, was isolated from subtropical forest soil of the Nanling National Forest Park located in Guangdong Province, P.R. China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FGD1 was most closely related to DSM 25049 (98.8 %), followed by DSM 25411 (98.7 %), DSM 32207 (98.2 %), DSM 22890 (98.1 %) and other species of (<98 %). The draft genome sequence was 4.58 Mb in length with a G+C content of 65.1 mol%. The calculated average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain FGD1 and closely related type strains were 77.7‒79.6 % and 21.7–22.9 %, respectively. Major fatty acids were summed feature 8 (C ω and/or C ω), summed feature 3 (C ω and/or C ω), C 2-OH and C. The predominant respiratory quinone was ubiquinone 10 and the major polyamine was spermidine. Polar lipids were composed of sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, an unidentified phospholipid and lipid. The polyphasic taxonomic results indicated that strain FGD1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FGD1 (=GDMCC 1.1761=KACC 21283).

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): Novosphingobium silvae , polyphasic taxonomy , subtropical forest soil and whole genome sequence
Funding
This study was supported by the:
  • the Science and Technology Planning Projects of Guangdong Province (Award 2018B030324001)
    • Principle Award Recipient: Guang-Da Feng
  • the Key Realm R&D Program of Guangdong Province (Award 2018B020205001)
    • Principle Award Recipient: Guang-Da Feng
  • the GDAS’ Special Project of Science and Technology Development (Award 2020GDASYL-20200302002)
    • Principle Award Recipient: Honghui Zhu
© 2020 The Authors
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2020-03-30
2025-01-15
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Novosphingobium silvae sp. nov., isolated from subtropical forest soil
Int J Syst Evol Microbiol 70, 2901 (2020); https://doi.org/10.1099/ijsem.0.004115
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