1887

Abstract

A novel Gram-stain-negative, aerobic and rod-shaped halophilic archaeon, designated HD8-45, was isolated from the red brine of salted brown alga produced at Dalian, PR China. According to the results of 16S rRNA gene and gene sequence comparisons, strain HD8-45 showed the highest sequence similarity to the corresponding genes of YGH44 (95.1 and 85.2 % similarities, respectively), EB27 (91.2 and 86.0 % similarities, respectively). The low sequence similarity and the phylogeny implied the novel generic status of strain HD8-45. Genomic relatedness analyses showed that strain HD8-45 were clearly distinguished from other species in the order , with average nucleotide identity, amino acid identity and DNA–DNA hybridization values not more than 75.1, 65.6 and 21.5 %. The polar lipid pattern contained phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two major glycolipids and two minor glycolipids. The two major glycolipids and a minor glycolipid were chromatographically identical to disulfated mannosyl glucosyl diether, sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively. The major respiratory quinones were menaquinone MK-8 and MK-8(H). The DNA G+C content was 62.0 mol% () and 61.9 mol% (genome). All these results showed that strain HD8-45 represents a novel species of a new genus in the order , for which the name gen. nov., sp. nov. is proposed. The type strain of is HD8-45 (=CGMCC 1.15328=JCM 31107).

Funding
This study was supported by the:
  • Heng-Lin Cui , National Science & Technology Infrastructure Program of China , (Award 2017FY100302)
  • Heng-Lin Cui , National Natural Science Foundation of China , (Award 31770005)
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2020-03-26
2020-06-03
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