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Volume 70, Issue 4

sp. nov., isolated from desert soil and reclassification of as a later heterotypic synonym of Free

Abstract

A novel actinobacterial strain, designated 13K301, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan. The taxonomic position of strain 13K301 was revealed by using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain 13K301 belongs to the genus and had highest sequence similarity to ‘’ S10 (99.2 %), NRRL B-16367 (98.9 %) and DSM 41896 (98.8 %), but the strain formed a distinct clade in the phylogenetic tree. The DNA–DNA relatedness and average nucleotide identity values as well as evolutionary distances based on multilocus (, , , and ) sequences between strain 13K301 and closely related type strains were significantly lower than the recommended threshold values. The cell wall contained -diaminopimelic acid and the whole-cell hydrolysates were glucose and ribose. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were determined as the predominant polar lipids. The major menaquinones were identified as MK-9(H) and MK-9(H). On the basis of these genotypic and phenotypic data, it is proposed that strain 13K301 should be classified as representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 13K301 (=DSM 106873=KCTC 49110). In addition, the whole genome-based comparisons as well as the multilocus sequence analysis revealed that the type strains of and belong to a single species. It is, therefore, proposed that be recognised as a heterotypic synonym of for which an emended description is given.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Actinobacteria , Karakum Desert , phylogenomic and Streptomyces
Funding
This study was supported by the:
  • Ondokuz Mayis Üniversitesi (Award PYO.FEN.1901.16.001)
    • Principle Award Recipient: Nevzat Sahin
© 2020 The Authors
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2020-03-16
2024-05-06
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Streptomyces cahuitamycinicus sp. nov., isolated from desert soil and reclassification of Streptomyces galilaeus as a later heterotypic synonym of Streptomyces bobili
Int J Syst Evol Microbiol 70, 2750 (2020); https://doi.org/10.1099/ijsem.0.004103
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