1887

Abstract

Three aerobic, rod-shaped actinobacterial strains, designated MMS17-SY117, MMS17-SY207-3 and MMS17-SY213, were isolated from soil and their taxonomic positions were analysed using a polyphasic approach. The isolates showed best growth at 30 °C, pH 7 and 0–1 % (w/v) NaCl. On the basis of 16S rRNA gene sequence similarity, the isolates were affiliated to the genus , and the closest species to MMS17-SY117, MMS17-SY207-3 and MMS17-SY213 were JC2056 (97.76%), IB-3 (97.41%) and RC825 (98.71%), respectively. Each isolate formed a distinct cluster within the clade in the phylogenetic tree. The orthologous average nucleotide identity and digital DNA–DNA hybridization values were in the range of 74.4–85.7 % and 16.6–39.2 %, respectively, with the type strains of related species. The major polar lipids in all three strains were phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol. The predominant fatty acids were iso-C and C ω8. MK-8(H) was the major isoprenoid quinone and -DAP was the major diamino acid. Galactose, glucose and rhamnose were present in the whole-cell hydrolysate, and MMS17-SY213 also contained mannose and ribose. The DNA G+C contents of MMS17-SY117, MMS17-SY207-3 and MMS17-SY213 were 72.2, 70.4 and 71.5 mol%, respectively. The phylogenetic, phenotypic and chemotaxonomic data supported the classification of each strain as representing a new species of , for which the names sp. nov. (MMS17-SY117=KCTC 49175=JCM 32831), sp. nov. (MMS17-SY207-3=KCTC 49176=JCM 32832) and sp. nov. (MMS17-SY213=KCTC 49177=JCM 32833) are proposed accordingly.

Funding
This study was supported by the:
  • Seung Bum Kim , National Institute of Biological Resources , (Award NA)
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2020-03-06
2020-06-04
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