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Volume 70, Issue 4

sp. nov., isolated from a fermentation process Free

Abstract

A novel Gram-stain-positive, aerobic, motile with peritrichous flagella, rod-shaped bacterium, designated CC-MHH1044, was isolated from a preserved vegetable sample. A polyphasic taxonomic approach was applied to the isolates in order to clarify its taxonomic position. Growth of the strain CC-MHH1044 occurred at 15–50 °C (optimum, 30 °C), pH 6.0–8.0 (optimum, pH 7.0) and with 0–2.0 % (w/v) NaCl (optimum, 1 %, w/v). The genome of strain CC-MHH1044 consisted of 8.5 Mb and the genomic DNA G+C content was 58.5 mol%. Comparison of the 16S rRNA gene sequences showed that CC-MHH1044 belonged to the genus and showed a close relationship with the type strains of (96.2 %) and (95.9 %), and lower sequence similarity to other species. Average nucleotide identity values calculated from whole-genome sequencing data proved that CC-MHH1044 represents a distinct species. The dominant cellular fatty acids (>5 %) included iso-C(7.4 %), iso-C (6.4 %), anteiso-C(40.3 %), C (6.6 %) and iso-C (27.0 %). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids, one unidentified phospholipid and glycolipid. The major polyamine was spermidine. The predominant isoprenoid quinone was menaqinone 7 (MK-7). Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits, together with results of comparative 16S rRNA gene sequence, average nucleotide identity and digital DNA–DNA hybridization analyses, we conclude that strain CC-MHH1044 represents a novel member of the genus , for which the name sp. nov. is proposed. The type strain is CC-MHH1044 (=BCRC 81147=JCM 32834).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): average nucleotide identity , Cohnella fermenti , polar lipid , polyamine and polyphasic taxonomic approach
Funding
This study was supported by the:
  • Ministry of Science and Technology, Taiwan (Award MOST 109-2634-F-005-002)
    • Principle Award Recipient: Chiu-Chung Young
  • Ministry of Science and Technology, Taiwan (Award MOST 109-2634-F-005-002)
    • Principle Award Recipient: Shih-Yao Lin
© 2020 The Authors
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2020-03-11
2024-05-14
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Cohnella fermenti sp. nov., isolated from a fermentation process
Int J Syst Evol Microbiol 70, 2602 (2020); https://doi.org/10.1099/ijsem.0.004080
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