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Volume 70, Issue 4

sp. nov., isolated from a deep well with oil reservoir water Free

Abstract

A Gram-stain-negative, non-motile and ovoid bacterial strain, designated 4-2, was isolated from oil-contaminated water which was collected from Xinjiang Province, north-west PR China. The 16S rRNA gene sequence analysis showed that strain 4-2 belonged to the genus . The species with highest similarity to strain 4-2 was YIM 90738 (97.83 %), followed by ‘’ M26 (97.83 %) and SYSUP0003 (97.25 %). The average nucleotide identity values between 4-2 and three type strains were 84.69, 77.88 and 74.07 %, respectively. The genomic DNA G+C content of strain 4-2 was 61.4 mol%. Chemotaxonomical characteristic results showed that the respiratory quinone was ubiquinone Q-10 and the major fatty acids were summed feature 8 (C 7 or C 6) and C cyclo 8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, unidentified phospholipids, an unidentified aminolipid and an unidentified polar lipid. The predominant polyamines were putrescine, cadaverine and spermidine. On the basis of phenotypic, chemotaxonomic and phylogenetic inferences, strain 4-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 4-2 (=CGMCC 1.13669=LMG 30882).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): average nucleotide identity , oil-contaminated water , Paracoccus alkanivorans sp. nov. and polyphasic taxonomy
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award Grant No. 41977198)
  • the sub-project of National Science and Technology Major Project (Award 2016ZX05040-002)
    • Principle Award Recipient: Lei Wang
  • Methodological research and application of oil field ecological profit and loss assessment (Award 2016E-1205)
    • Principle Award Recipient: Lei Wang
© 2020 The Authors
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2020-02-10
2024-04-27
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Paracoccus alkanivorans sp. nov., isolated from a deep well with oil reservoir water
Int J Syst Evol Microbiol 70, 2312 (2020); https://doi.org/10.1099/ijsem.0.004036
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