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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 4

gen. nov., sp. nov., a novel bacterium isolated from a hot spring, and proposal of fam. nov. Free

Abstract

A novel thermotolerant bacterial strain was isolated from a hot spring in a Tibetan geothermal field. Phylogenetic analysis of the 16S rRNA gene sequence of the novel strain showed that it belongs to a distinct lineage far from any known taxa. The new isolate shared the highest pairwise sequence identity with S-12 (92.8 % similarity) according to the 16S rRNA gene sequences. Cells were Gram-stain-negative, aerobic, rod-shaped and formed white round colonies. The strain grew at the ranges of 28–45 °C (optimum, 37 °C), pH 5.0–7.0 (optimum, pH 6.0) and 0–2 % NaCl. The strain was positive for catalase and oxidase. The major respiratory quinone was ubiquinone Q-10. Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were summed feature 8 (C ω7 and/or C ω6). The DNA G+C content was 68.3 mol%. Based on these distinguishing properties, this strain is proposed to represent a new species of a new genus gen. nov., sp. nov., within a new family fam. nov. The type strain of the type species of is SYSU G02060 (=KCTC 72351=CGMCC 1.17070).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): hot spring , polyphasic taxonomy , Rhabdaerophilum calidifontis. gen. nov. , sp. nov. and Tibetan geothermal field
Funding
This study was supported by the:
  • Science and Technology Program of Guangzhou, China (Award 201803030030)
    • Principle Award Recipient: Wen-Jun Li
  • National Natural Science Foundation of China (Award 31670009)
    • Principle Award Recipient: Wen-Jun Li
  • National Natural Science Foundation of China (Award 91951205)
    • Principle Award Recipient: Wen-Jun Li
© 2020 Sun Yat-Sen University, PR China
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Rhabdaerophilum calidifontis gen. nov., sp. nov., a novel bacterium isolated from a hot spring, and proposal of Rhabdaerophilaceae fam. nov.
Int J Syst Evol Microbiol 70, 2298 (2020); https://doi.org/10.1099/ijsem.0.004035
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