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Volume 70, Issue 4

sp. nov., isolated from the intestinal tract of a Korean indigenous freshwater fish, Free

Abstract

A novel Gram-negative, obligately aerobic, rod-shaped and non-motile bacterium, designated strain K13M18, was isolated from the intestinal tract of a Korean indigenous fish, oily bitterling (). Strain K13M18 formed creamy-pink colonies on a marine agar plate. Results of phylogenetic analysis based on the 16S rRNA gene sequence similarity indicated that strain K13M18 was most closely related to DRYC-M-16, sharing 97.62 % similarity with that strain. Strain K13M18 belonged to the genus , which formed a cluster with RCRI19, SY72, DRYC-M-16 and DJC in a phylogenetic tree based on the 16S rRNA gene sequences. Strain K13M18 grown optimally in 0 % (w/v) NaCl, at pH 7 and 30 °C, in a marine broth medium. The predominant cellular fatty acids were C 7 and C 6. The major respiratory isoprenoid quinone was ubiquinone Q-10. Polar lipids of strain K13M18 contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, six unidentified aminophospholipids, one unidentified aminolipid and an unidentified lipid. Based on genome sequencing, the DNA G+C content of strain K13M18 was 64.08 mol %, with an average nucleotide identity value, calculated by a comparative genomic analysis of strains K13M18 and DRYC-M-16, of 74.82 %. Based on the phylogenetic, genotypic, and phenotypic information, strain K13M18 is proposed to be a novel species of the genus . The type strain is K13M18 (=KCTC 62659=JCM 33230).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Acheilognathus koreensis , gut microbiota , Korean indigenous freshwater fish and Tabrizicola piscis
Funding
This study was supported by the:
  • National Research Foundation of Korea (Award NRF-2018R1A5A1025077)
    • Principle Award Recipient: Jin-Woo Bae
  • Ministry of Environment (MOE) of the Republic of Korea (Award NIBR201801106)
    • Principle Award Recipient: Jin-Woo Bae
  • Ministry of Science, ICT and Future Planning (Award NRF-2017M3A9F3046549)
    • Principle Award Recipient: Jin-Woo Bae
  • Ministry of Science, ICT and Future Planning (Award NRF-2016R1E1A1A02921587)
    • Principle Award Recipient: Jin-Woo Bae
  • Ministry of Science, ICT and Future Planning (Award NRF-2015M3C9A2054299)
    • Principle Award Recipient: Jin-Woo Bae
© 2020 The Authors
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2020-02-10
2024-05-04
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Tabrizicola piscis sp. nov., isolated from the intestinal tract of a Korean indigenous freshwater fish, Acheilognathus koreensis
Int J Syst Evol Microbiol 70, 2305 (2020); https://doi.org/10.1099/ijsem.0.004034
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