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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 4

sp. nov., a pesticide-degrading bacterium isolated from the insect living in deserted cropland Free

Abstract

A Gram-stain-negative, non-spore-forming, motile, aerobic, rod-shaped bacteria strain, designated LCB8, was isolated from the insect captured from a deserted cropland in Shuangliu district, Chengdu, PR China. Phylogenetic analysis on the basis of 16S rRNA gene sequence indicated that the strain represented a member of the genus , family , class CCUG 60088 (97.9 %) and CCUG 38531 (98.8 %) were identified as the most closely related phylogenetic neighbours of strain LCB8. The novel strain was able to grow at salt concentrations of 0–4.5 % (w/v), pH 5–9 and temperatures of 20–42 °C. The major quinone system was ubiquinone Q-10, the major fatty acids were Cω7, C and C. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and four undefined aminolipids. The major polyamines were putrescine and spermidine. Genome sequencing revealed a genome size of 4.76 Mbp and a DNA G+C content of 57.1 mol%. These phenotypic, genotypic and chemotaxonomic traits excellently supported the affiliation of LCB8 to the genus . Pairwise determined whole-genome average nucleotide identity (ANI) values indicated that strain LCB8 represents a novel species, for which we propose the name sp. nov. with the type strain LCB8 (=KCTC 72031=CGMCC 1.13984).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): genome , Ochrobactrum teleogrylli , phylogenetic analysis and Teleogryllus occipitalis
Funding
This study was supported by the:
  • the National Key Research and Development Program of China (Award 2018YFC1802201)
    • Principle Award Recipient: Yongqiang Tian
© 2020 The Authors
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2020-02-25
2024-05-04
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Ochrobactrum teleogrylli sp. nov., a pesticide-degrading bacterium isolated from the insect Teleogryllus occipitalis living in deserted cropland
Int J Syst Evol Microbiol 70, 2217 (2020); https://doi.org/10.1099/ijsem.0.003964
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