1887

Abstract

A novel bacterial strain, XY-99, was isolated from the epidermis of a marine invertebrate of the genus from seawater of the South China Sea. The cells of the strain were aerobic, Gram-stain-negative, non-motile, and oval-shaped (0.8–1.0 µm wide and 1.0–1.5 µm long) without a flagellum. The strain grew at temperatures of 15–37 °C (optimum, 35–37 °C), at pH 5.5–9.5 (optimum 7.5), and at NaCl concentrations of 0–12.0 % (w/v) (optimum 1.5–3.0 %). The major fatty acids (>10 %) were summed feature 8 (comprising Cω7 and/or Cω6), C and 11-methyl Cω7. The predominant polar lipid was diphosphatidylglycerol. The respiratory quinone was Q-10. The closet phylogenetic neighbours were DY470 and JLT1210, showing 97.5 and 97.3 % of 16 s rRNA gene sequence similarity. The genome size of XY-99 was 3 673 499 bp, with 64.5 % DNA G+C content. The average nucleotide identity and digital DNA–DNA hybridization values between XY-99 and DY470 were 72.8 and 14.0 %, respectively, while they were 79.3 and 22.3 % between XY-99 and JLT1210. Characterization based on phylogenetic, phenotypic, chemotaxonomic and genomic evidence demonstrated that XY-99 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XY-99 (=KCTC 72211=MCCC 1K03607).

Funding
This study was supported by the:
  • Shenzhen science and technology application demonstration project (Award KJYY20180201180253571)
    • Principle Award Recipient: Ying Xu
  • the Scientific and Technical Innovation Council of Shenzhen (Award KQJSCX20170727101743831)
    • Principle Award Recipient: Ying Xu
  • the 62st-class General Financial Grant from the China Postdoctoral Science Foundation (Award No. 2017M622754)
    • Principle Award Recipient: Qi Yin
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2019-11-28
2021-07-29
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