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Abstract

A bacterial strain designated FSY-9 was isolated from a freshwater mesocosm in Taiwan and characterized to determine its taxonomic affiliation. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain FSY-9 formed a phylogenetic lineage in the genus . Strain FSY-9 was most closely related to R1-4 with a 97.2 % 16S rRNA gene sequence similarity. Strain FSY-9 showed 71.3–72.6 % average nucleotide identity and 17.7–23.0 % digital DNA–DNA hybridization identity with the strains of other species. Cells of strain FSY-9 were facultatively anaerobic, Gram-negative, rod-shaped, non-motile and formed light yellow coloured colonies. Growth occurred at 15–37 °C and pH 5.5–7, and in the presence of 0–0.5 % NaCl. The major fatty acids (>10 %) of strain FSY-9 were summed feature 3 (Cω7 and/or Cω6), Cω7 and C. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine, sphingoglycolipid, an uncharacterized aminophospholipid, an uncharacterized glycolipid and an uncharacterized lipid. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 61.5 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain FSY-9 should be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FSY-9 (=BCRC 81052=LMG 30054=KCTC 52813).

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2019-12-05
2020-01-24
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