1887

Abstract

A total of 21 yeast isolates were recovered as part of a research project on biodiversity of yeasts in traditional dairy products in Alborz province, Iran. Standard protocols were used to carry out phenotypic, biochemical, physiological characterization and the phylogenetic analysis of combined the D1/D2 domain of the large ribosomal subunit (26S or LSU) and ITS region sequences. Five strains represented a potential new ascomycetous yeast species. Ascospore formation was not observed in these strains, and they did not ferment the examined carbon sources. Phylogenetic analysis placed these isolates in a well-supported sub-clade in the genus . Here, we describe this novel yeast as sp. nov.

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2019-11-20
2021-08-04
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References

  1. Iranmanesh M, Ezzatpanah H, Akbari-Adergani B, Karimi Torshizi MA. SPME/GC-MS characterization of volatile compounds of Iranian traditional dried Kashk. Int J Food Prop 2018; 21:1067–1079 [View Article]
    [Google Scholar]
  2. Soltani M, Güzeler N. The production and quality properties of liquid Kashks. GIDA / J Food 2013; 38:1–7
    [Google Scholar]
  3. Kivsnҫ M, Funda EG. A functional food: a traditional Tarhana fermentation. Food Sci Technol 2017; 37:269–274
    [Google Scholar]
  4. Hesselbart A, Junker K, Wendland J. Draft genome sequence of Saccharomycopsis fermentans CBS 7830, a predacious yeast belonging to the Saccharomycetales . Genome Announc 2018; 11:pii: e01445–17
    [Google Scholar]
  5. Suh S-O, Blackwell M, Kurtzman CP, Lachance M-A. Phylogenetics of Saccharomycetales, the ascomycete yeasts. Mycologia 2006; 98:1006–1017 [View Article]
    [Google Scholar]
  6. Schiӧnning H. Nouveau genre de la famille des Saccharomyce` tes . Compt Rend Trav Lab Carlsberg 1903; 6:101–125
    [Google Scholar]
  7. Kurtzman CP, Boekhout T, Fell JW. (editors) The Yeasts, a Taxonomic Study 2, 5th ed. London: Elsevier; 2011
    [Google Scholar]
  8. Jacques N, Louis-Mondesir C, Coton M, Coton E, Casaregola S. Two novel Saccharomycopsis species isolated from black olive brines and a tropical plant. Description of Saccharomycopsis olivae f. a., sp. nov. and Saccharomycopsis guyanensis f. a., sp. nov. Reassignment of Candida amapae to Saccharomycopsis amapae f. a., comb. nov., Candida lassenensis to Saccharomycopsis lassenensis f. a., comb. nov. and Arthroascus babjevae to Saccharomycopsis babjevae f. a., comb. nov. Int J Syst Evol Microbiol 2014; 64:2169–2175 [View Article]
    [Google Scholar]
  9. Kurtzman CP, Robnett CJ. Molecular relationships among hyphal ascomycetous yeasts and yeastlike taxa. Can J Bot 1995; 73:824–830 [View Article]
    [Google Scholar]
  10. Yamada Y, Matsuda M, Mikata K. The phylogeny of species of the genus Saccharomycopsis Schiӧnning (Saccharomycetaceae) based on the partial sequences of 18S and 26S ribosomal RNAs. Biosci Biotechnol Biochem 1996; 60:1303–1307 [View Article]
    [Google Scholar]
  11. Naumov GI, Naumova ES, Smith MT, de Hoog GS. Ribosomal DNA sequencing and reinstatement of the genus Arthroascus von Arx. J Gen Appl Microbiol 2003; 49:267–270 [View Article]
    [Google Scholar]
  12. Morais PB, Rosa CA, Meyer SA. Mendonc a-Hagler LC, Hagler A N. Candida amapae, a new amino acid-requiring yeast from Amazonian fruit, Parahancornia amapa . J Ind Microbiol 1995; 14:531–535
    [Google Scholar]
  13. Kurtzman CP. Two new members of the Saccharomycopsis clade: Saccharomycopsis microspora comb. nov. and Candida lassenensis, sp. nov. Mycotaxon 1999; 71:241–250
    [Google Scholar]
  14. Groenewald M, Lombard L, de Vries M, Lopez AG, Smith M et al. Diversity of yeast species from Dutch garden soil and the description of six novel Ascomycetes. FEMS Yeast Res 2018; 18: [View Article]
    [Google Scholar]
  15. Barnett JA, Payne RW, Yarrow D. Yeasts: Characteristics and Identification, 3rd ed. Cambridge University Press; 2000
    [Google Scholar]
  16. Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study 1, 5th ed. Elsevier; 2011 pp 87–110
    [Google Scholar]
  17. Hanna M, Xiao W. Xiao W. editor Yeast Protocols (Methods in Molecular Biology) Humana Press; 2006 pp 15–20
    [Google Scholar]
  18. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications San Diego, USA: Academic Press; 1990 pp 315–322
    [Google Scholar]
  19. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article]
    [Google Scholar]
  20. Lachance MA, Rosa CA, Carvajal EJ, Freitas LFD, Bowles JM et al. Saccharomycopsis fodiens sp., nov., a rare predacious yeast from three distant localities. Int J Syst Evol Microbiol 2012; 62:2793–2798
    [Google Scholar]
  21. Katoh K, Rozewicki J, Yamada KD. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 2017bbx108
    [Google Scholar]
  22. Silvestro D, Michalak I. raxmlGUI: a graphical front-end for RAxML. Org Divers Evol 2012; 12:335–337 [View Article]
    [Google Scholar]
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