1887

Abstract

A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2, was isolated from red soil in Jiangxi Province, PR China. The strain grew at 25–45 °C and at pH 4.0–7.0, and was able to tolerate up to 50 mM Zn. The complete genome of strain SCAWS-G2 was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4–0.8 % differences). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2 formed a distinct lineage within the order , showing <89.2 % sequence similarities to the recognized taxa of this order. The whole-genome based phylogenomic tree separated strain SCAWS-G2 from the recognized families within . The genome-wide average nucleotide identity values between strain SCAWS-G2 and the related type strains were <68.2 %. The strain can also be differentiated from the recognized families by a number of phenotypic characteristics. The polar lipids of SCAWS-G2 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, seven unidentified glycolipids and one unidentified lipid. The peptidoglycan amino acids contained ornithine, glycine, glutamic acid and alanine, and the cell-wall sugars were mainly galactose and rhamnose. The major fatty acids were C 2-OH, C and iso-C. Based on all these data, we propose that strain SCAWS-G2 represents a novel genus and species, gen. nov., sp. nov., within the new family fam. nov. of the order . The type strain of is SCAWS-G2 (=CGMCC 1.16132=DSM 105258).

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2019-11-15
2019-12-09
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