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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 2

gen. nov., sp. nov., a novel representative of the class , isolated from red soil, and proposal of fam. nov. Free

Abstract

A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2, was isolated from red soil in Jiangxi Province, PR China. The strain grew at 25–45 °C and at pH 4.0–7.0, and was able to tolerate up to 50 mM Zn. The complete genome of strain SCAWS-G2 was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4–0.8 % differences). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2 formed a distinct lineage within the order , showing <89.2 % sequence similarities to the recognized taxa of this order. The whole-genome based phylogenomic tree separated strain SCAWS-G2 from the recognized families within . The genome-wide average nucleotide identity values between strain SCAWS-G2 and the related type strains were <68.2 %. The strain can also be differentiated from the recognized families by a number of phenotypic characteristics. The polar lipids of SCAWS-G2 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, seven unidentified glycolipids and one unidentified lipid. The peptidoglycan amino acids contained ornithine, glycine, glutamic acid and alanine, and the cell-wall sugars were mainly galactose and rhamnose. The major fatty acids were C 2-OH, C and iso-C. Based on all these data, we propose that strain SCAWS-G2 represents a novel genus and species, gen. nov., sp. nov., within the new family fam. nov. of the order . The type strain of is SCAWS-G2 (=CGMCC 1.16132=DSM 105258).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): complete genome sequence , Ktedonosporobacter , Ktedonosporobacteraceae , polyphasic taxonomy and red soil
Funding
This study was supported by the:
  • National Natural Science Foundation of China (NSFC) (Award 31700008)
    • Principle Award Recipient: Minghao Liu
  • National Natural Science Foundation of China (NSFC) (Award 31670502)
    • Principle Award Recipient: Ying Huang
© 2020 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.003864
2019-11-15
2025-01-24
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Ktedonosporobacter rubrisoli gen. nov., sp. nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam. nov.
Int J Syst Evol Microbiol 70, 1015 (2020); https://doi.org/10.1099/ijsem.0.003864
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