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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 2

gen. nov., sp. nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of fam. nov. in the order Free

Abstract

A novel bacterium, strain Seoho-28, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically. Strain Seoho-28 was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium. The strain grew optimally with 0 % NaCl and at 25–30 °C on Reasoner's 2A medium. The phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the order , but sequence similarities to known species were less than 94.7 %. The genomic DNA G+C content of the strain Seoho-28 was 74.2 mol%. Genomic comparisons of strain Seoho-28 with families in the order were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively). Strain Seoho-28 contained C-iso, C 9 and C as major fatty acids and MK-7 (H) as the major quinone. Strain Seoho-28 contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids. - and -diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan. Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28 represents a novel genus and species, gen. nov., sp. nov., which belongs to a new family in the order and the class . The type strain is Seoho-28 (=KCTC 39791=JCM 31881).

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Paraconexibacter , Paraconexibacter algicola , Paraconexibacteraceae and polyphasic taxonomy
Funding
This study was supported by the:
  • Ministry of Education, Science and Technology (Award 2015H1D3A1060001)
    • Principle Award Recipient: Yingshun Cui
  • Ministry of Education, Science and Technology (Award 2016M1A5A1027453)
    • Principle Award Recipient: Hee-Mock Oh
© 2020 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.003846
2019-11-07
2025-01-20
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Paraconexibacter algicola gen. nov., sp. nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam. nov. in the order Solirubrobacterales
Int J Syst Evol Microbiol 70, 915 (2020); https://doi.org/10.1099/ijsem.0.003846
/content/journal/ijsem/10.1099/ijsem.0.003846
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