1887

Abstract

A novel, Gram-stain-negative, motile, flagellated, aerobic, rod-shaped (0.5–0.7 µm wide and 1.0–1.2 µm long) and faint-yellow strain, designated ALS 84, was isolated from marine sediment sampled at Ailian bay, Rongcheng, PR China. Growth occurred in the presence of 1–3 % (w/v) NaCl (optimum, 2 % NaCl), at pH 4.0–8.0 (pH 6.0–7.0) and at 8–30 °C (28 °C). The genome size was 4.37 Mbp. The G+C content of the genomic DNA was 33.6 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences suggested that strain ALS 84 belongs to the genus within the family , and is most closely related to (95.6 % similarity). The major fatty acids (>10 %) were iso-C 3-OH (22.9 %), iso-C (14.0 %) and C (10.9 %). The major polar lipids were phosphatidylethanolamine and three unidentified lipids. Menaquinone-6 (MK-6) was identified as the respiratory quinone. On the basis of the phenotypic, phylogenetic and chemotaxonomic data obtained in the study, strain ALS 84 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the novel species is strain ALS 84 (=KCTC 62398=MCCC 1K03480).

Funding
This study was supported by the:
  • Zenghu Zhang , key lab of marine bioactive substance and modern analytical technique, SOA , (Award MBSMAT-2018-02)
  • Yongyu Zhang , the senior user’s project of RV KEXUE , (Award KEXUE2018G02)
  • Yongyu Zhang , QIBEBT and Dalian National Laboratory For Clean Energy (DNL), CAS , (Award QIBEBT ZZBS 201805)
  • Zenghu Zhang , Natural Science Foundation of China , (Award 31700104)
  • Jihua Liu , Natural Science Foundation of China , (Award 41876174)
  • Yongyu Zhang , Natural Science Foundation of China , (Award 41606134)
  • Yongyu Zhang , open task of Qingdao National Laboratory for Marine Science and Technology , (Award QNLM2016ORP0311)
  • Hongmei Li , National Key Research and Development Programs of China , (Award 2016YFC1402101-03)
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2019-11-12
2020-10-27
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