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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 2

sp. nov., isolated from marine sediment Free

Abstract

A novel, Gram-stain-negative, motile, flagellated, aerobic, rod-shaped (0.5–0.7 µm wide and 1.0–1.2 µm long) and faint-yellow strain, designated ALS 84, was isolated from marine sediment sampled at Ailian bay, Rongcheng, PR China. Growth occurred in the presence of 1–3 % (w/v) NaCl (optimum, 2 % NaCl), at pH 4.0–8.0 (pH 6.0–7.0) and at 8–30 °C (28 °C). The genome size was 4.37 Mbp. The G+C content of the genomic DNA was 33.6 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences suggested that strain ALS 84 belongs to the genus within the family , and is most closely related to (95.6 % similarity). The major fatty acids (>10 %) were iso-C 3-OH (22.9 %), iso-C (14.0 %) and C (10.9 %). The major polar lipids were phosphatidylethanolamine and three unidentified lipids. Menaquinone-6 (MK-6) was identified as the respiratory quinone. On the basis of the phenotypic, phylogenetic and chemotaxonomic data obtained in the study, strain ALS 84 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the novel species is strain ALS 84 (=KCTC 62398=MCCC 1K03480).

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Bacteroidetes , Flavicella sediminum , marine sediment and sp. nov.
Funding
This study was supported by the:
  • key lab of marine bioactive substance and modern analytical technique, SOA (Award MBSMAT-2018-02)
    • Principle Award Recipient: Zenghu Zhang
  • the senior user’s project of RV KEXUE (Award KEXUE2018G02)
    • Principle Award Recipient: Yongyu Zhang
  • QIBEBT and Dalian National Laboratory For Clean Energy (DNL), CAS (Award QIBEBT ZZBS 201805)
    • Principle Award Recipient: Yongyu Zhang
  • Natural Science Foundation of China (Award 31700104)
    • Principle Award Recipient: Zenghu Zhang
  • Natural Science Foundation of China (Award 41876174)
    • Principle Award Recipient: Jihua Liu
  • Natural Science Foundation of China (Award 41606134)
    • Principle Award Recipient: Yongyu Zhang
  • open task of Qingdao National Laboratory for Marine Science and Technology (Award QNLM2016ORP0311)
    • Principle Award Recipient: Yongyu Zhang
  • National Key Research and Development Programs of China (Award 2016YFC1402101-03)
    • Principle Award Recipient: Hongmei Li
© 2020 The Authors
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2024-04-26
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Flavicella sediminum sp. nov., isolated from marine sediment
Int J Syst Evol Microbiol 70, 868 (2020); https://doi.org/10.1099/ijsem.0.003839
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