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A Gram-stain-positive actinobacterium, designated strain GSSD-12T, was isolated from an alpine wetland soil. blast search of the full-length 16S rRNA gene sequence of GSSD-12T indicated it represented a member of the genus Streptomyces , and displayed highest similarity with Streptomyces scopuliridis NRRL B-24574T (99.2 %) and less than 98.6 % similarity with other species of the genus Streptomyces with validly published names. Phylogenetic analysis based on 16S rRNA gene sequences indicated that GSSD-12T was closely related to S. scopuliridis NRRL B-24574T, Streptomyces odonnellii NRRL B-24891T and Streptomyces lushanensis NRRL B-24994T. However, the digital DNA–DNA hybridization value, the average nucleotide identity value and the multilocus sequence analysis evolutionary distance between this strain and its closest relatives indicated that it represented a distinct species. Furthermore, GSSD-12T was also distinctly differentiated from them by morphological, physiological and biochemical characteristics. Therefore, strain GSSD-12T(=CICC 11051T=JCM 33019T) represents a novel species of the genus Streptomyces , for which the name Streptomyces paludis sp. nov. is proposed.
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