1887

Abstract

A Gram-stain negative, aerobic, motile and rod-shaped bacterium, designated strain 3.1105, was isolated from a karst district soil sample collected from Tiandong cave, Guizhou province, south-west PR China. The isolate grew at 10–40 °C and pH 5.0–8.0 and tolerated up to 1 % NaCl (w/v) on R2A medium, with optimal growth at 25–30 °C, pH 7.0 and 0 % NaCl (w/v). Cells showed oxidase-positive and catalase-positive reactions. The respiratory quinone was Q-10. The predominant cellular fatty acids contained Cω7 11-methyl, summed feature 8 (Cω7 or Cω6), C and C. The major polar lipids were phosphatidylglycerol and monoglycosyldiglycerides. The genomic DNA G+C content was 56.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that 3.1105 should be affiliated to the genus and showed highest 16S rRNA gene sequence similarity values with CB 48 (96.0 %), ZFGT-14 (95.3 %) and lower than 95.3 % similarity to other species of the genus . The polyphasic taxonomic characteristics indicated that strain 3.1105 represents a novel species of the genus , for which the name sp. nov., (type strain 3.1105=KCTC 62978=CCTCC AB 2018268) is proposed.

Funding
This study was supported by the:
  • the National Key R&D Program of China (Award No. 2017YFD0201104)
    • Principle Award Recipient: Ming-He Mo
  • Yunnan Provincial Science and Technology Department (CN) (Award No. 2018BC001)
    • Principle Award Recipient: Ming-He Mo
  • the Department of Science and Technology of Yunnan Province (Award No. 2017FA016)
    • Principle Award Recipient: Ming-He Mo
  • China Tobacco Yunnan Industrial Corp (CN) (Award No. 2019530000241018)
    • Principle Award Recipient: Ming-He Mo
  • China Construction Eighth Engineering Division (CN) (Award No.2018530000241019)
    • Principle Award Recipient: Ming-He Mo
  • National Outstanding Youth Science Fund Project of National Natural Science Foundation of China (CN) (Award No. 31570108)
    • Principle Award Recipient: Ming-He Mo
  • National Natural ScienceFoundation of China (Award No.31870091)
    • Principle Award Recipient: Ming-He Mo
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2021-10-28
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