1887

Abstract

A novel Gram-reaction-negative, facultative-aerobic, motile, non-spore-forming, oval-shaped and denitrifying bacterium, designated BO-81, was isolated from sludge sampled in the Republic of Korea. This bacterium was investigated via a polyphasic approach to reveal its taxonomic position. The results of phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain BO-81 belonged to the family and was related to the closest species (98.8 % sequence similarity), ‘’ (98.4 %), (97.8 %), (96.8 %) and (96.2 %). The average nucleotide identity and DNA–DNA hybridization values between strain BO-81 and its closely related strains were 77.7–94.2 % and 20.1–55.9 %, respectively, indicating that BO-81 represents a novel species of the genus . Growth occurred at 18–40 °C on Reasoner's 2A medium in the presence of 0–7 % NaCl (w/v) and at pH 6.0–9.0. Strain BO-81 was characterized chemotaxonomically as having ubiquinone 10 as its predominant respiratory quinone, summed feature 8 (C 6 and/or C 7) as its major fatty acid and phosphatidylglycerol and phosphatidylethanolamine as its predominant polar lipids. The G+C content of the genomic DNA was 68.3 mol%. The results of physiological and biochemical tests allowed phenotypic differentiation of strain BO-81 from other species with validly published names. Therefore, the isolate represented a novel species, for which the name sp. nov. (type strain BO-81=KACC 19677=LMG 30808) is proposed.

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2019-10-30
2019-11-17
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