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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 1

sp. nov., an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand Free

Abstract

A Gram-stain-positive, aerobic bacterium, designated CPCC 204705, was isolated from a desert soil sample, collected from the Badain Jaran desert. Growth of strain CPCC 204705 was observed at pH 6.0–8.0 and 15–37 °C, with optimal growth at 28 °C and pH 7.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705 belonged to the genus , showing the highest similarity (98.54 %) of 16S rRNA gene sequence to JCM 17534. The peptidoglycan type was A4, containing -ornithine and d-glutamic acids as diagnostic amino acids. Rhamnose and galactose were detected in the whole-cell hydrolysate as diagnostic sugars. The major cellular fatty acids were anteiso-C, anteiso-CA, C and C. The major menaquinone was MK-9 (H) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids. The DNA–DNA hybridization value between strain CPCC 204705 and JCM 17534 was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %. The DNA G+C content of strain CPCC 204705 was 75.4 mol%. Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA–DNA hybridization value, strain CPCC 204705 should be classified as a novel species. The name sp. nov. is proposed, with strain CPCC 204705 (=DSM 105430=KCTC 39974) as the type strain.

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Cellulomonas telluris , genome and polyphasic taxonomy
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31670010)
    • Principle Award Recipient: Yu-Qin Zhang
© 2020 The Authors
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2020-02-03
2024-05-14
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Cellulomonas telluris sp. nov., an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand
Int J Syst Evol Microbiol 70, 631 (2020); https://doi.org/10.1099/ijsem.0.003806
/content/journal/ijsem/10.1099/ijsem.0.003806
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