1887

Abstract

A Gram-stain-negative, yellow-green bacterium, designated 1.1416, was isolated from wormcast of . The strain was non-motile, rod-shaped, and grew optimally on NA medium at 30 °C, pH 7.0 and with 0 % (w/v) NaCl. On the basis of the 16S rRNA gene sequence and phylogenetic analysis, 1.1416 showed the highest degree of 16S rRNA gene sequence similarity to 26-35 (96.2 %), followed by G3 (96.1 %). The respiratory quinone of 1.1416 was ubiquinone-8 (Q-8), and its major cellular fatty acids were iso-C (39.8 %), summed feature 9 (iso-C ω9 or C 10-methyl) (18.6 %). The major polar lipids of 1.1416 were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and six unidentified phospholipids. The genomic DNA G+C content of 1.1416 was 71.0 mol%. According to the results of the phenotypic and chemotaxonomic phylogenetic analyses, strain 1.1416 represents a novel species of the genus , for which the name sp. nov. is proposed, with strain 1.1416 (=KCTC 62979=CCTCC AB 2018348) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003799
2019-11-07
2019-11-13
Loading full text...

Full text loading...

References

  1. Finkmann W, Altendorf K, Stackebrandt E, Lipski A. Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. Int J Syst Evol Microbiol 2000;50: 273– 282 [CrossRef]
    [Google Scholar]
  2. Mu Y, Pan Y, Shi W, Liu L, Jiang Z et al. Luteimonas arsenica sp. nov., an arsenic-tolerant bacterium isolated from arsenic-contaminated soil. Int J Syst Evol Microbiol 2016;66: 2291– 2296 [CrossRef]
    [Google Scholar]
  3. Ngo HTT, Yin CS. Luteimonas terrae sp. nov., isolated from rhizosphere soil of Radix ophiopogonis. Int J Syst Evol Microbiol 2016;66: 1920– 1925 [CrossRef]
    [Google Scholar]
  4. Lin SY, Hameed A, Shahina M, Liu YC, Hsu YH et al. Description of Luteimonas pelagia sp. nov., isolated from marine sediment, and emended descriptions of Luteimonas aquatica, Luteimonas composti, Luteimonas mephitis, Lysobacter enzymogenes and Lysobacter panaciterrae. Int J Syst Evol Microbiol 2016;66: 645– 651 [CrossRef]
    [Google Scholar]
  5. Zhao GY, Shao F, Zhang M, Zhang XJ, Wang JY et al. Luteimonas rhizosphaerae sp. nov., isolated from the rhizosphere of Triticum aestivum L. Int J Syst Evol Microbiol 2018;68: 1197 1203 [CrossRef]
    [Google Scholar]
  6. Young CC, Kämpfer P, Chen WM, Yen WS, Arun AB et al. Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste. Int J Syst Evol Microbiol 2007;57: 741– 744 [CrossRef]
    [Google Scholar]
  7. Park YJ, Park MS, Lee SH, Park W, Lee K et al. Luteimonas lutimaris sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2011;61: 2729– 2733 [CrossRef]
    [Google Scholar]
  8. Artursson V, Jansson JK. Use of bromodeoxyuridine immunocapture to identify active bacteria associated with arbuscular mycorrhizal hyphae. Appl Environ Microbiol 2003;69: 6208– 6215 [CrossRef]
    [Google Scholar]
  9. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62: 716– 721 [CrossRef]
    [Google Scholar]
  10. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4: 406– 425 [CrossRef]
    [Google Scholar]
  11. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17: 368– 376 [CrossRef]
    [Google Scholar]
  12. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20: 406– 416 [CrossRef]
    [Google Scholar]
  13. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33: 1870– 1874 [CrossRef]
    [Google Scholar]
  14. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16: 111– 120 [CrossRef]
    [Google Scholar]
  15. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39: 783– 791 [CrossRef]
    [Google Scholar]
  16. Xi L, Zhang Z, Qiao N, Zhang Y, Li J et al. Complete genome sequence of the novel thermophilic polyhydroxyalkanoates producer Aneurinibacillus sp. XH2 isolated from Gudao oilfield in China. J Biotechnol 2016;227: 54– 55 [CrossRef]
    [Google Scholar]
  17. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018;68: 461– 466 [CrossRef]
    [Google Scholar]
  18. Skerman VBD. A Guide to the Identification of the Genera of Bacteria, 2nd ed. Baltimore: Williams & Wilkins; 1967
    [Google Scholar]
  19. Gregersen T. Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eru J Appl Microbiol Biotechnol 1978;5: 123– 127 [CrossRef]
    [Google Scholar]
  20. Luo X, Wang J, Zeng XC, Wang Y, Zhou L et al. Mycetocola manganoxydans sp. nov., an actinobacterium isolated from the Taklamakan desert. Int J Syst Evol Microbiol 2012;62: 2967– 2970 [CrossRef]
    [Google Scholar]
  21. Smibert RM, Krieg NR. Phenotypic characterization In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington: American Society for Microbiology; 1994; pp 607– 655
    [Google Scholar]
  22. Xu P, Li W-J, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005;55: 1149– 1153 [CrossRef]
    [Google Scholar]
  23. Fraser SL, Jorgensen JH. Reappraisal of the antimicrobial susceptibilities of Chryseobacterium and Flavobacterium species and methods for reliable susceptibility testing. Antimicrob Agents Chemother 1997;41: 2738– 2741 [CrossRef]
    [Google Scholar]
  24. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  25. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 1979;47: 87– 95 [CrossRef]
    [Google Scholar]
  26. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2: 233– 241 [CrossRef]
    [Google Scholar]
  27. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19: 161– 207
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003799
Loading
/content/journal/ijsem/10.1099/ijsem.0.003799
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error