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Abstract

A Gram-stain-negative, yellow-green bacterium, designated 1.1416, was isolated from wormcast of . The strain was non-motile, rod-shaped, and grew optimally on NA medium at 30 °C, pH 7.0 and with 0 % (w/v) NaCl. On the basis of the 16S rRNA gene sequence and phylogenetic analysis, 1.1416 showed the highest degree of 16S rRNA gene sequence similarity to 26-35 (96.2 %), followed by G3 (96.1 %). The respiratory quinone of 1.1416 was ubiquinone-8 (Q-8), and its major cellular fatty acids were iso-C (39.8 %), summed feature 9 (iso-C ω9 or C 10-methyl) (18.6 %). The major polar lipids of 1.1416 were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and six unidentified phospholipids. The genomic DNA G+C content of 1.1416 was 71.0 mol%. According to the results of the phenotypic and chemotaxonomic phylogenetic analyses, strain 1.1416 represents a novel species of the genus , for which the name sp. nov. is proposed, with strain 1.1416 (=KCTC 62979=CCTCC AB 2018348) as the type strain.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award No.31570108)
    • Principle Award Recipient: Ming He Mo
  • National Natural Science Foundation of China (Award No.31870091)
    • Principle Award Recipient: Ming He Mo
  • China Tobacco Yunnan Industrial Co. Ltd. (Award No.2018530000241019)
    • Principle Award Recipient: Ming He Mo
  • China Tobacco Yunnan Industrial Co. Ltd. (Award No. 2019530000241018)
    • Principle Award Recipient: Ming He Mo
  • the Department of Science and Technology of Yunnan Province (Award No. 2017FA016)
    • Principle Award Recipient: Ming He Mo
  • Yunnan Provincial Science and Technology Department (CN) (Award No. 2018BC001)
    • Principle Award Recipient: Ming He Mo
  • National Key R&D Program of China (Award No. 2017YFD0201104)
    • Principle Award Recipient: Ming He Mo
  • This research was supported by the projects from National Natural Science Foundation of China (Award No. 31960022)
    • Principle Award Recipient: Ming He Mo
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2020-02-03
2021-08-04
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