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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 1

sp. nov., a moderately halophilic bacterium isolated from a marine solar saltern Free

Abstract

A facultatively anaerobic, Gram-stain-negative and non-gliding bacterium, designated F01, was isolated from marine solar saltern in Weihai, PR China. Cells of F01 were 0.2–0.4 µm wide and 1.4–4.1 µm long, weakly catalase-positive and oxidase-negative. Growth of F01 was determined to occur at 4–40 °C (optimum, 33–37 °C), pH 6.5–8.5 (optimum, 7.0–8.0), and with 0.5–18.0 % (w/v) NaCl (optimum, 3.0–6.0 %). The 16S rRNA gene sequence analysis indicated that F01 represented a member of the genus within the family . Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was most closely related to DSM 16394, with a sequence similarity of 97.5 %. The DNA G+C content of the isolate was 57.6 mol%. The major respiratory quinone of F01 was ubiquinone-9 (Q-9) and the major fatty acids were anteiso-C, C and Cω9. The major polar lipids were phosphoaminolipid, phosphatidylglycerol and phosphatidylethanolamine. On the basis of the results of the phylogenetic analysis and phenotypic properties, it is concluded that F01 can be considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is F01 (=MCCC 1H00290=KCTC 52700).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16SrRNA gene , Marinobacter and phylogenetic analysis
Funding
This study was supported by the:
  • Science and Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: Zong-Jun Du
  • National Natural Science Foundation of China (Award 31770002)
    • Principle Award Recipient: Zong-Jun Du
© 2020 The Authors
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2019-10-08
2024-04-26
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Marinobacter vulgaris sp. nov., a moderately halophilic bacterium isolated from a marine solar saltern
Int J Syst Evol Microbiol 70, 450 (2020); https://doi.org/10.1099/ijsem.0.003774
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