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Volume 70, Issue 1

sp. nov., isolated from paddy soil, and reclassification of (Zhao . 2017) as comb. nov. Free

Abstract

A polyphasic taxonomic approach was used to characterize a nitrogen-fixing bacterium, designated strain CC-HIH110, isolated from paddy soil in Taiwan. Cells of strain CC-HIH110 were Gram-stain-negative, rod-shaped, motile with polar flagella, catalase-positive and oxidase-positive. Optimal growth occurred at 30 °С, pH 7 and 1 % NaCl. Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-HIH110 associated with (98.4 % sequence identity), (97.8 %), (97.7 %) and (96.0 %), and lower sequence similarity to other species. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain CC-HIH110 and the type strains of other closely related species were 71.5–88.6 % and 19.6–35.5 %, respectively. Strain CC-HIH110 contained C 3-OH, C 3-OH/iso C I and C 7/C 6 as the predominant fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine, three unknown aminophospholipids, two unknown phospholipids and an unknown lipid. The major polyamine was homospermidine. The DNA G+C content was 55.0 mol% and the predominant quinone was ubiquinone (Q-10). Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence, ANI and dDDH analyses, strain CC-HIH110 is proposed to represent a novel species, for which the name sp. nov. (type strain CC-HIH110=BCRC 80932=JCM 31228). In addition, is reclassified as (type strain N19=ACCC 19962=KCTC 52413) comb. nov.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Allorhizobium terrae , average nucleotide identity , digital DNA–DNA hybridization and strain CC-HIH110T
Funding
This study was supported by the:
  • Ministry of Science and Technology, Taiwan, http://dx.doi.org/10.13039/501100004663 (Award MOST 108-2634-F-005-002)
  • Ministry of Science and Technology, Taiwan, http://dx.doi.org/10.13039/501100004663 (Award MOST 108-2634-F-005-002)
© 2020 The Authors
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Allorhizobium terrae sp. nov., isolated from paddy soil, and reclassification of Rhizobium oryziradicis (Zhao et al. 2017) as Allorhizobium oryziradicis comb. nov.
Int J Syst Evol Microbiol 70, 397 (2020); https://doi.org/10.1099/ijsem.0.003770
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